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3j6s

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Revision as of 20:18, 9 December 2016

Cryo-EM structure of Dengue virus serotype 3 at 28 degrees C

Structural highlights

3j6s is a 6 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	3j6t, 3j6u
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[Q6DLV0_9FLAV] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS000336_004_099774]

Publication Abstract from PubMed

Dengue virus (DENV) infects ~400 million people annually. There is no licensed vaccine or therapeutic drug. Only a small fraction of the total DENV-specific antibodies in a naturally occurring dengue infection consists of highly neutralizing antibodies. Here we show that the DENV-specific human monoclonal antibody 5J7 is exceptionally potent, neutralizing 50% of virus at nanogram-range antibody concentration. The 9 A resolution cryo-electron microscopy structure of the Fab 5J7-DENV complex shows that a single Fab molecule binds across three envelope proteins and engages three functionally important domains, each from a different envelope protein. These domains are critical for receptor binding and fusion to the endosomal membrane. The ability to bind to multiple domains allows the antibody to fully coat the virus surface with only 60 copies of Fab, that is, half the amount compared with other potent antibodies. Our study reveals a highly efficient and unusual mechanism of molecular recognition by an antibody.

A highly potent human antibody neutralizes dengue virus serotype 3 by binding across three surface proteins.,Fibriansah G, Tan JL, Smith SA, de Alwis R, Ng TS, Kostyuchenko VA, Jadi RS, Kukkaro P, de Silva AM, Crowe JE, Lok SM Nat Commun. 2015 Feb 20;6:6341. doi: 10.1038/ncomms7341. PMID:25698059^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fibriansah G, Tan JL, Smith SA, de Alwis R, Ng TS, Kostyuchenko VA, Jadi RS, Kukkaro P, de Silva AM, Crowe JE, Lok SM. A highly potent human antibody neutralizes dengue virus serotype 3 by binding across three surface proteins. Nat Commun. 2015 Feb 20;6:6341. doi: 10.1038/ncomms7341. PMID:25698059 doi:http://dx.doi.org/10.1038/ncomms7341

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3j6s"

@@ Line 1: / Line 1: @@
 ==Cryo-EM structure of Dengue virus serotype 3 at 28 degrees C==
 <StructureSection load='3j6s' size='340' side='right' caption='[[3j6s]], [[Resolution|resolution]] 6.00&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[3j6s]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J6S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J6S FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j6t|3j6t]], [[3j6u|3j6u]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j6s RCSB], [http://www.ebi.ac.uk/pdbsum/3j6s PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6s OCA], [http://pdbe.org/3j6s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j6s RCSB], [http://www.ebi.ac.uk/pdbsum/3j6s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j6s ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3j6s" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

3j6s

From Proteopedia

Revision as of 20:18, 9 December 2016

Cryo-EM structure of Dengue virus serotype 3 at 28 degrees C

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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