3ux4

From Proteopedia

(Difference between revisions)

Revision as of 19:02, 10 December 2016

Crystal structure of the urea channel from the human gastric pathogen Helicobacter pylori

Structural highlights

3ux4 is a 3 chain structure with sequence from Campylobacter pylori j99. The February 2013 RCSB PDB Molecule of the Month feature on Proton-Gated Urea Channel by David Goodsell is 10.2210/rcsb_pdb/mom_2013_2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	jhp_0066, ureI (Campylobacter pylori J99)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[UREI_HELPJ] Functions as a specific, H(+)-activated urea channel that increases the rate of urea entry into the cytoplasm, resulting in activation of cytoplasmic urease at acidic medium pH. Is essential for H.pylori gastric survival and colonization. Is necessary for the adaptation of urease activity to the extracellular pH, as in the presence of urea, UreI rapidly enhances the production of ammonia in the extracellular medium when the pH of the medium was decreased to pH5 or below (By similarity).

Publication Abstract from PubMed

Half the world's population is chronically infected with Helicobacter pylori, causing gastritis, gastric ulcers and an increased incidence of gastric adenocarcinoma. Its proton-gated inner-membrane urea channel, HpUreI, is essential for survival in the acidic environment of the stomach. The channel is closed at neutral pH and opens at acidic pH to allow the rapid access of urea to cytoplasmic urease. Urease produces NH(3) and CO(2), neutralizing entering protons and thus buffering the periplasm to a pH of roughly 6.1 even in gastric juice at a pH below 2.0. Here we report the structure of HpUreI, revealing six protomers assembled in a hexameric ring surrounding a central bilayer plug of ordered lipids. Each protomer encloses a channel formed by a twisted bundle of six transmembrane helices. The bundle defines a previously unobserved fold comprising a two-helix hairpin motif repeated three times around the central axis of the channel, without the inverted repeat of mammalian-type urea transporters. Both the channel and the protomer interface contain residues conserved in the AmiS/UreI superfamily, suggesting the preservation of channel architecture and oligomeric state in this superfamily. Predominantly aromatic or aliphatic side chains line the entire channel and define two consecutive constriction sites in the middle of the channel. Mutation of Trp 153 in the cytoplasmic constriction site to Ala or Phe decreases the selectivity for urea in comparison with thiourea, suggesting that solute interaction with Trp 153 contributes specificity. The previously unobserved hexameric channel structure described here provides a new model for the permeation of urea and other small amide solutes in prokaryotes and archaea.

Structure of the proton-gated urea channel from the gastric pathogen Helicobacter pylori.,Strugatsky D, McNulty R, Munson K, Chen CK, Soltis SM, Sachs G, Luecke H Nature. 2012 Dec 9. doi: 10.1038/nature11684. PMID:23222544^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Strugatsky D, McNulty R, Munson K, Chen CK, Soltis SM, Sachs G, Luecke H. Structure of the proton-gated urea channel from the gastric pathogen Helicobacter pylori. Nature. 2012 Dec 9. doi: 10.1038/nature11684. PMID:23222544 doi:http://dx.doi.org/10.1038/nature11684

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3ux4"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the urea channel from the human gastric pathogen Helicobacter pylori==
 <StructureSection load='3ux4' size='340' side='right' caption='[[3ux4]], [[Resolution|resolution]] 3.26&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3ux4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori_j99 Helicobacter pylori j99]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UX4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3ux4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_pylori_j99 Campylobacter pylori j99]. The February 2013 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Proton-Gated Urea Channel''  by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2013_2 10.2210/rcsb_pdb/mom_2013_2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UX4 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=XP4:1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHATE'>XP4</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">jhp_0066, ureI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85963 Helicobacter pylori J99])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">jhp_0066, ureI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85963 Campylobacter pylori J99])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ux4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ux4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ux4 RCSB], [http://www.ebi.ac.uk/pdbsum/3ux4 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ux4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ux4 OCA], [http://pdbe.org/3ux4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ux4 RCSB], [http://www.ebi.ac.uk/pdbsum/3ux4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ux4 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/UREI_HELPJ UREI_HELPJ]] Functions as a specific, H(+)-activated urea channel that increases the rate of urea entry into the cytoplasm, resulting in activation of cytoplasmic urease at acidic medium pH. Is essential for H.pylori gastric survival and colonization. Is necessary for the adaptation of urease activity to the extracellular pH, as in the presence of urea, UreI rapidly enhances the production of ammonia in the extracellular medium when the pH of the medium was decreased to pH5 or below (By similarity).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 15: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3ux4" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 22: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Helicobacter pylori j99]]
+[[Category: Campylobacter pylori j99]]
+[[Category: Proton-Gated Urea Channel]]
+[[Category: RCSB PDB Molecule of the Month]]
 [[Category: Luecke, H]]
 [[Category: McNulty, R]]
 [[Category: Transport protein]]

3ux4

From Proteopedia

Revision as of 19:02, 10 December 2016

Crystal structure of the urea channel from the human gastric pathogen Helicobacter pylori

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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