1gr0

From Proteopedia

(Difference between revisions)

Revision as of 14:22, 22 December 2016

myo-inositol 1-phosphate synthase from Mycobacterium tuberculosis in complex with NAD and zinc.

Structural highlights

1gr0 is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Activity:	Inositol-3-phosphate synthase, with EC number 5.5.1.4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

[INO1_MYCTU] Catalyzes the conversion of glucose 6-phosphate to 1D-myo-inositol 3-phosphate.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Phosphatidylinositol (PI) is essential for Mycobacterium tuberculosis viability and the enzymes involved in the PI biosynthetic pathway are potential antimycobacterial agents for which little structural information is available. The rate-limiting step in the pathway is the production of (L)-myo-inositol 1-phosphate from (D)-glucose 6-phosphate, a complex reaction catalyzed by the enzyme inositol 1-phosphate synthase. We have determined the crystal structure of this enzyme from Mycobacterium tuberculosis (tbINO) at 1.95 A resolution, bound to the cofactor NAD+. The active site is located within a deep cleft at the junction between two domains. The unexpected presence of a zinc ion here suggests a mechanistic difference from the eukaryotic inositol synthases, which are stimulated by monovalent cations, that may be exploitable in developing selective inhibitors of tbINO.

Crystal structure of inositol 1-phosphate synthase from Mycobacterium tuberculosis, a key enzyme in phosphatidylinositol synthesis.,Norman RA, McAlister MS, Murray-Rust J, Movahedzadeh F, Stoker NG, McDonald NQ Structure. 2002 Mar;10(3):393-402. PMID:12005437^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Norman RA, McAlister MS, Murray-Rust J, Movahedzadeh F, Stoker NG, McDonald NQ. Crystal structure of inositol 1-phosphate synthase from Mycobacterium tuberculosis, a key enzyme in phosphatidylinositol synthesis. Structure. 2002 Mar;10(3):393-402. PMID:12005437

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1gr0"

@@ Line 1: / Line 1: @@
 ==myo-inositol 1-phosphate synthase from Mycobacterium tuberculosis in complex with NAD and zinc.==
 <StructureSection load='1gr0' size='340' side='right' caption='[[1gr0]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1gr0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GR0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GR0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1gr0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GR0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GR0 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inositol-3-phosphate_synthase Inositol-3-phosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.4 5.5.1.4] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gr0 OCA], [http://pdbe.org/1gr0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gr0 RCSB], [http://www.ebi.ac.uk/pdbsum/1gr0 PDBsum], [http://www.topsan.org/Proteins/TBSGC/1gr0 TOPSAN]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gr0 OCA], [http://pdbe.org/1gr0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gr0 RCSB], [http://www.ebi.ac.uk/pdbsum/1gr0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gr0 ProSAT], [http://www.topsan.org/Proteins/TBSGC/1gr0 TOPSAN]</span></td></tr>
 </table>
 == Function ==

1gr0

From Proteopedia

Revision as of 14:22, 22 December 2016

myo-inositol 1-phosphate synthase from Mycobacterium tuberculosis in complex with NAD and zinc.

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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