1r0n
From Proteopedia
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|PDB= 1r0n |SIZE=350|CAPTION= <scene name='initialview01'>1r0n</scene>, resolution 2.60Å | |PDB= 1r0n |SIZE=350|CAPTION= <scene name='initialview01'>1r0n</scene>, resolution 2.60Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= RXRA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), ECR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster]) | |GENE= RXRA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), ECR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1r0o|1R0O]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r0n OCA], [http://www.ebi.ac.uk/pdbsum/1r0n PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1r0n RCSB]</span> | ||
}} | }} | ||
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[[Category: Ozyhar, A.]] | [[Category: Ozyhar, A.]] | ||
[[Category: Rastinejad, F.]] | [[Category: Rastinejad, F.]] | ||
| - | [[Category: ZN]] | ||
[[Category: dna binding domain]] | [[Category: dna binding domain]] | ||
[[Category: ecdysone receptor]] | [[Category: ecdysone receptor]] | ||
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[[Category: ultraspiracle]] | [[Category: ultraspiracle]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:21:34 2008'' |
Revision as of 20:21, 30 March 2008
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| , resolution 2.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Gene: | RXRA (Homo sapiens), ECR (Drosophila melanogaster) | ||||||
| Related: | 1R0O
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Heterodimeric Ecdsyone receptor DNA binding complex
Overview
Ecdysteroids initiate molting and metamorphosis in insects via a heterodimeric receptor consisting of the ecdysone receptor (EcR) and ultraspiracle (USP). The EcR-USP heterodimer preferentially mediates transcription through highly degenerate pseudo-palindromic response elements, resembling inverted repeats of 5'-AGGTCA-3' separated by 1 bp (IR-1). The requirement for a heterodimeric arrangement of EcR-USP subunits to bind to a symmetric DNA is unusual within the nuclear receptor superfamily. We describe the 2.24 A structure of the EcR-USP DNA-binding domain (DBD) heterodimer bound to an idealized IR-1 element. EcR and USP use similar surfaces, and rely on the deformed minor groove of the DNA to establish protein-protein contacts. As retinoid X receptor (RXR) is the mammalian homolog of USP, we also solved the 2.60 A crystal structure of the EcR-RXR DBD heterodimer on IR-1 and found the dimerization and DNA-binding interfaces to be the same as in the EcR-USP complex. Sequence alignments indicate that the EcR-RXR heterodimer is an important model for understanding how the FXR-RXR heterodimer binds to IR-1 sites.
About this Structure
1R0N is a Protein complex structure of sequences from Drosophila melanogaster and Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the heterodimeric ecdysone receptor DNA-binding complex., Devarakonda S, Harp JM, Kim Y, Ozyhar A, Rastinejad F, EMBO J. 2003 Nov 3;22(21):5827-40. PMID:14592980
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