1r16
From Proteopedia
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|PDB= 1r16 |SIZE=350|CAPTION= <scene name='initialview01'>1r16</scene>, resolution 2.0Å | |PDB= 1r16 |SIZE=350|CAPTION= <scene name='initialview01'>1r16</scene>, resolution 2.0Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=PYF:3-PYRIDINYLCARBINOL'>PYF</scene>, <scene name='pdbligand=ROB:1-DEOXY-RIBOFURANOSE-5'-PHOSPHATE'>ROB</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/NAD(+)_nucleosidase NAD(+) nucleosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.5 3.2.2.5] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)_nucleosidase NAD(+) nucleosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.5 3.2.2.5] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1lbe|1LBE]], [[1ism|1ISM]], [[1isj|1ISJ]], [[1isi|1ISI]], [[1ish|1ISH]], [[1isg|1ISG]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r16 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r16 OCA], [http://www.ebi.ac.uk/pdbsum/1r16 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1r16 RCSB]</span> | ||
}} | }} | ||
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[[Category: Szebenyi, D M.E.]] | [[Category: Szebenyi, D M.E.]] | ||
[[Category: Thiel, D J.]] | [[Category: Thiel, D J.]] | ||
| - | [[Category: PYF]] | ||
| - | [[Category: ROB]] | ||
[[Category: adp-ribosyl cyclase]] | [[Category: adp-ribosyl cyclase]] | ||
[[Category: ca2+ signalling]] | [[Category: ca2+ signalling]] | ||
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[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:21:47 2008'' |
Revision as of 20:21, 30 March 2008
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| , resolution 2.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | NAD(+) nucleosidase, with EC number 3.2.2.5 | ||||||
| Related: | 1LBE, 1ISM, 1ISJ, 1ISI, 1ISH, 1ISG
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Aplysia ADP ribosyl cyclase with bound pyridylcarbinol and R5P
Overview
ADP-ribosyl cyclase catalyzes the elimination of nicotinamide from NAD and cyclization to cADPR, a known second messenger in cellular calcium signaling pathways. We have determined to 2.0 A resolution the structure of Aplysia cyclase with ribose-5-phosphate bound covalently at C3' and with the base exchange substrate (BES), pyridylcarbinol, bound to the active site. In addition, further refinement at 2.4 A resolution of the structure of nicotinamide-bound cyclase, which was previously reported, reveals that ribose-5-phosphate is also covalently bound in this structure, and a second nicotinamide site was identified. The structures of native and mutant Glu179Ala cyclase were also solved to 1.7 and 2.0 A respectively. It is proposed that the second nicotinamide site serves to promote cyclization by clearing the active site of the nicotinamide byproduct. Moreover, a ribosylation mechanism can be proposed in which the cyclization reaction proceeds through a covalently bound intermediate.
About this Structure
1R16 is a Single protein structure of sequence from Aplysia californica. Full crystallographic information is available from OCA.
Reference
ADP-ribosyl cyclase; crystal structures reveal a covalent intermediate., Love ML, Szebenyi DM, Kriksunov IA, Thiel DJ, Munshi C, Graeff R, Lee HC, Hao Q, Structure. 2004 Mar;12(3):477-86. PMID:15016363
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