4ldd

From Proteopedia

(Difference between revisions)

Revision as of 07:13, 23 December 2016

Crystal Structure of Ebola virus VP40 Hexamer

Structural highlights

4ldd is a 3 chain structure with sequence from Zebov. The October 2014 RCSB PDB Molecule of the Month feature on Ebola Virus Proteins by David Goodsell is 10.2210/rcsb_pdb/mom_2014_10. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	4ld8, 4ldb, 4ldi, 4ldm
Gene:	VP40 (ZEBOV)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[VP40_EBOZM] Promotes virus assembly and budding by interacting with host proteins of the multivesicular body pathway. May facilitate virus budding by interacting with the nucleocapsid and the plasma membrane. Specific interactions with membrane-associated GP and VP24 during the budding process may also occur. The hexamer form seems to be involved in budding. The octamer form binds RNA, and may play a role in genome replication.^[1] ^[2]

Publication Abstract from PubMed

Proteins, particularly viral proteins, can be multifunctional, but the mechanisms behind multifunctionality are not fully understood. Here, we illustrate through multiple crystal structures, biochemistry, and cellular microscopy that VP40 rearranges into different structures, each with a distinct function required for the ebolavirus life cycle. A butterfly-shaped VP40 dimer traffics to the cellular membrane. Once there, electrostatic interactions trigger rearrangement of the polypeptide into a linear hexamer. These hexamers construct a multilayered, filamentous matrix structure that is critical for budding and resembles tomograms of authentic virions. A third structure of VP40, formed by a different rearrangement, is not involved in virus assembly but instead uniquely binds RNA to regulate viral transcription inside infected cells. These results provide a functional model for ebolavirus matrix assembly and the other roles of VP40 in the virus life cycle and demonstrate how a single wild-type, unmodified polypeptide can assemble into different structures for different functions. PAPERFLICK:

Structural Rearrangement of Ebola Virus VP40 Begets Multiple Functions in the Virus Life Cycle.,Bornholdt ZA, Noda T, Abelson DM, Halfmann P, Wood MR, Kawaoka Y, Saphire EO Cell. 2013 Aug 15;154(4):763-74. doi: 10.1016/j.cell.2013.07.015. PMID:23953110^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Irie T, Licata JM, Harty RN. Functional characterization of Ebola virus L-domains using VSV recombinants. Virology. 2005 Jun 5;336(2):291-8. PMID:15892969 doi:10.1016/j.virol.2005.03.027
↑ Johnson RF, Bell P, Harty RN. Effect of Ebola virus proteins GP, NP and VP35 on VP40 VLP morphology. Virol J. 2006 May 23;3:31. PMID:16719918 doi:10.1186/1743-422X-3-31
↑ Bornholdt ZA, Noda T, Abelson DM, Halfmann P, Wood MR, Kawaoka Y, Saphire EO. Structural Rearrangement of Ebola Virus VP40 Begets Multiple Functions in the Virus Life Cycle. Cell. 2013 Aug 15;154(4):763-74. doi: 10.1016/j.cell.2013.07.015. PMID:23953110 doi:10.1016/j.cell.2013.07.015

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4ldd"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Ebola virus VP40 Hexamer==
 <StructureSection load='4ldd' size='340' side='right' caption='[[4ldd]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ld8|4ld8]], [[4ldb|4ldb]], [[4ldi|4ldi]], [[4ldm|4ldm]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VP40 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186538 ZEBOV])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ldd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ldd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ldd RCSB], [http://www.ebi.ac.uk/pdbsum/4ldd PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ldd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ldd OCA], [http://pdbe.org/4ldd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ldd RCSB], [http://www.ebi.ac.uk/pdbsum/4ldd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ldd ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4ldd" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4ldd

From Proteopedia

Revision as of 07:13, 23 December 2016

Crystal Structure of Ebola virus VP40 Hexamer

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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