1r5u
From Proteopedia
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|PDB= 1r5u |SIZE=350|CAPTION= <scene name='initialview01'>1r5u</scene>, resolution 4.50Å | |PDB= 1r5u |SIZE=350|CAPTION= <scene name='initialview01'>1r5u</scene>, resolution 4.50Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span> |
|GENE= Sua7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |GENE= Sua7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1i6h|1I6h]], [[1pft|1pft]], [[1dl6|1dl6]], [[1i3q|1I3q]], [[1i5o|1I5o]], [[1nik|1NIK]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r5u OCA], [http://www.ebi.ac.uk/pdbsum/1r5u PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1r5u RCSB]</span> | ||
}} | }} | ||
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[[Category: Kornberg, R D.]] | [[Category: Kornberg, R D.]] | ||
[[Category: Westover, K D.]] | [[Category: Westover, K D.]] | ||
| - | [[Category: MG]] | ||
| - | [[Category: ZN]] | ||
[[Category: zinc ribbon]] | [[Category: zinc ribbon]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:23:40 2008'' |
Revision as of 20:23, 30 March 2008
| |||||||
| , resolution 4.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | Sua7 (Saccharomyces cerevisiae) | ||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||
| Related: | 1I6h, 1pft, 1dl6, 1I3q, 1I5o, 1NIK
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RNA POLYMERASE II TFIIB COMPLEX
Overview
The structure of the general transcription factor IIB (TFIIB) in a complex with RNA polymerase II reveals three features crucial for transcription initiation: an N-terminal zinc ribbon domain of TFIIB that contacts the "dock" domain of the polymerase, near the path of RNA exit from a transcribing enzyme; a "finger" domain of TFIIB that is inserted into the polymerase active center; and a C-terminal domain, whose interaction with both the polymerase and with a TATA box-binding protein (TBP)-promoter DNA complex orients the DNA for unwinding and transcription. TFIIB stabilizes an early initiation complex, containing an incomplete RNA-DNA hybrid region. It may interact with the template strand, which sets the location of the transcription start site, and may interfere with RNA exit, which leads to abortive initiation or promoter escape. The trajectory of promoter DNA determined by the C-terminal domain of TFIIB traverses sites of interaction with TFIIE, TFIIF, and TFIIH, serving to define their roles in the transcription initiation process.
About this Structure
1R5U is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structural basis of transcription: an RNA polymerase II-TFIIB cocrystal at 4.5 Angstroms., Bushnell DA, Westover KD, Davis RE, Kornberg RD, Science. 2004 Feb 13;303(5660):983-8. PMID:14963322
Page seeded by OCA on Sun Mar 30 23:23:40 2008
