1r9w
From Proteopedia
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|LIGAND= | |LIGAND= | ||
|ACTIVITY= | |ACTIVITY= | ||
| - | |GENE= E1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | + | |GENE= E1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=333761 Human papillomavirus type 18]) |
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9w OCA], [http://www.ebi.ac.uk/pdbsum/1r9w PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1r9w RCSB]</span> | ||
}} | }} | ||
| Line 16: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1R9W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 1R9W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_papillomavirus_type_18 Human papillomavirus type 18]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9W OCA]. |
==Reference== | ==Reference== | ||
The DNA-binding domain of human papillomavirus type 18 E1. Crystal structure, dimerization, and DNA binding., Auster AS, Joshua-Tor L, J Biol Chem. 2004 Jan 30;279(5):3733-42. Epub 2003 Oct 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14593106 14593106] | The DNA-binding domain of human papillomavirus type 18 E1. Crystal structure, dimerization, and DNA binding., Auster AS, Joshua-Tor L, J Biol Chem. 2004 Jan 30;279(5):3733-42. Epub 2003 Oct 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14593106 14593106] | ||
| - | [[Category: Human papillomavirus type | + | [[Category: Human papillomavirus type 18]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Auster, A S.]] | [[Category: Auster, A S.]] | ||
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[[Category: viral replication]] | [[Category: viral replication]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:25:10 2008'' |
Revision as of 20:25, 30 March 2008
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| , resolution 1.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | E1 (Human papillomavirus type 18) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the DNA-binding domain of the human papillomavirus type 18 (HPV-18) replication initiation protein E1
Overview
High risk types of human papillomavirus, such as type 18 (HPV-18), cause cervical carcinoma, one of the most frequent causes of cancer death in women worldwide. DNA replication is one of the central processes in viral maintenance, and the machinery involved is an excellent target for the design of antiviral therapy. The papillomaviral DNA replication initiation protein E1 has origin recognition and ATP-dependent DNA melting and helicase activities, and it consists of a DNA-binding domain and an ATPase/helicase domain. While monomeric in solution, E1 binds DNA as a dimer. Dimerization occurs via an interaction of hydrophobic residues on a single alpha-helix of each monomer. Here we present the crystal structure of the monomeric HPV-18 E1 DNA-binding domain refined to 1.8-A resolution. The structure reveals that the dimerization helix is significantly different from that of bovine papillomavirus type 1 (BPV-1). However, we demonstrate that the analogous residues required for E1 dimerization in BPV-1 and the low risk HPV-11 are also required for HPV-18 E1. We also present evidence that the HPV-18 E1 DNA-binding domain does not share the same nucleotide and amino acid requirements for specific DNA recognition as BPV-1 and HPV-11 E1.
About this Structure
1R9W is a Single protein structure of sequence from Human papillomavirus type 18. Full crystallographic information is available from OCA.
Reference
The DNA-binding domain of human papillomavirus type 18 E1. Crystal structure, dimerization, and DNA binding., Auster AS, Joshua-Tor L, J Biol Chem. 2004 Jan 30;279(5):3733-42. Epub 2003 Oct 30. PMID:14593106
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