5mji
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of RosB with bound intermediate OHC-RP (8-demethyl-8-formylriboflavin-5'-phosphate)== | |
| + | <StructureSection load='5mji' size='340' side='right' caption='[[5mji]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5mji]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MJI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MJI FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7O6:[(2~{S},3~{R},4~{R})-5-[8-METHANOYL-7-METHYL-2,4-BIS(OXIDANYLIDENE)-1~{H}-BENZO[G]PTERIDIN-10-IUM-10-YL]-2,3,4-TRIS(OXIDANYL)PENTYL]+DIHYDROGEN+PHOSPHATE'>7O6</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mji OCA], [http://pdbe.org/5mji PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mji RCSB], [http://www.ebi.ac.uk/pdbsum/5mji PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mji ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | 8-demethyl-8-aminoriboflavin-5'-phosphate (AFP) synthase (RosB) catalyzes the key reaction of roseoflavin biosynthesis by forming AFP from riboflavin-5'-phosphate (RP) and glutamate via the intermediates 8-demethyl-8-formylriboflavin-5'-phosphate (OHC-RP) and 8-demethyl-8-carboxylriboflavin-5'-phosphate (HO2 C-RP). To understand this reaction in which a methyl substituent of an aromatic ring is replaced by an amine we structurally characterized RosB in complex with OHC-RP (2.0 A) and AFP (1.7 A). RosB is composed of four flavodoxin-like subunits which have been upgraded with specific extensions and a unique C-terminal arm. It appears that RosB has evolved from an electron- or hydride-transferring flavoprotein to a sophisticated multi-step enzyme which uses RP as a substrate (and not as a cofactor). Structure-based active site analysis was complemented by mutational and isotope-based mass-spectrometric data to propose an enzymatic mechanism on an atomic basis. | ||
| - | + | The Crystal Structure of RosB: Insights into the Reaction Mechanism of the First Member of a Family of Flavodoxin-like Enzymes.,Konjik V, Brunle S, Demmer U, Vanselow A, Sandhoff R, Ermler U, Mack M Angew Chem Int Ed Engl. 2016 Dec 16. doi: 10.1002/anie.201610292. PMID:27981706<ref>PMID:27981706</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5mji" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bruenle, S]] | ||
| + | [[Category: Demmer, U]] | ||
| + | [[Category: Ermler, U]] | ||
| + | [[Category: Konjik, V]] | ||
| + | [[Category: Mack, M]] | ||
| + | [[Category: Sandhoff, R]] | ||
| + | [[Category: Vanselow, A]] | ||
| + | [[Category: Electron transport]] | ||
| + | [[Category: Flavodoxin-like]] | ||
| + | [[Category: Flavoprotein]] | ||
| + | [[Category: Roseoflavin]] | ||
Current revision
Crystal Structure of RosB with bound intermediate OHC-RP (8-demethyl-8-formylriboflavin-5'-phosphate)
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