1rjv
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1rjv |SIZE=350|CAPTION= <scene name='initialview01'>1rjv</scene> | |PDB= 1rjv |SIZE=350|CAPTION= <scene name='initialview01'>1rjv</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= PVALB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= PVALB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rjv OCA], [http://www.ebi.ac.uk/pdbsum/1rjv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rjv RCSB]</span> | ||
}} | }} | ||
| Line 30: | Line 33: | ||
[[Category: Quattrone, A.]] | [[Category: Quattrone, A.]] | ||
[[Category: SPINE, Structural Proteomics in Europe.]] | [[Category: SPINE, Structural Proteomics in Europe.]] | ||
| - | [[Category: CA]] | ||
[[Category: calcium]] | [[Category: calcium]] | ||
[[Category: ef-hand]] | [[Category: ef-hand]] | ||
| Line 40: | Line 42: | ||
[[Category: structural proteomics in europe]] | [[Category: structural proteomics in europe]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:29:11 2008'' |
Revision as of 20:29, 30 March 2008
| |||||||
| Ligands: | |||||||
| Gene: | PVALB (Homo sapiens) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution Structure of Human alpha-Parvalbumin refined with a paramagnetism-based strategy
Overview
In the frame of a research aimed at the detailed structural characterization of human calcium-binding proteins of the EF-hand family, the solution structure of human alpha-parvalbumin has been solved by NMR and refined with the help of substitution of the Ca(2+) ion in the EF site with the paramagnetic Dy(3+) ion. A simple (1)H-(15)N HSQC spectrum allowed the NH assignments based on the properties of Dy(3+). This allowed us to exploit pseudocontact shifts and residual dipolar couplings for solution structure refinement. The backbone and heavy atom RMSD are 0.55 +/- 0.08 and 1.02 +/- 0.08 A, respectively, and decrease to 0.39 +/- 0.05 and 0.90 +/- 0.06 A upon refinement with paramagnetism-based restraints. The RMSD for the metal itself in the EF site in the refined structure is 0.26 +/- 0.12 A. Backbone NH R(1), R(2), and NOE measured at two temperatures show the protein to be relatively rigid. The NH orientations are well determined by the paramagnetism-based restraints. This allows us to detect small but significant local structural differences with the orthologue protein from rat, whose X-ray structure is available at 2.0 A resolution. All differences are related to local changes in the amino acidic composition.
About this Structure
1RJV is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Paramagnetism-based refinement strategy for the solution structure of human alpha-parvalbumin., Baig I, Bertini I, Del Bianco C, Gupta YK, Lee YM, Luchinat C, Quattrone A, Biochemistry. 2004 May 11;43(18):5562-73. PMID:15122922
Page seeded by OCA on Sun Mar 30 23:29:11 2008
