1rpe
From Proteopedia
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|PDB= 1rpe |SIZE=350|CAPTION= <scene name='initialview01'>1rpe</scene>, resolution 2.500Å | |PDB= 1rpe |SIZE=350|CAPTION= <scene name='initialview01'>1rpe</scene>, resolution 2.500Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rpe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rpe OCA], [http://www.ebi.ac.uk/pdbsum/1rpe PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rpe RCSB]</span> | ||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1RPE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 1RPE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Phage_434 Phage 434]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RPE OCA]. |
==Reference== | ==Reference== | ||
The phage 434 OR2/R1-69 complex at 2.5 A resolution., Shimon LJ, Harrison SC, J Mol Biol. 1993 Aug 5;232(3):826-38. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8355273 8355273] | The phage 434 OR2/R1-69 complex at 2.5 A resolution., Shimon LJ, Harrison SC, J Mol Biol. 1993 Aug 5;232(3):826-38. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8355273 8355273] | ||
| - | [[Category: | + | [[Category: Phage 434]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Harrison, S C.]] | [[Category: Harrison, S C.]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:31:13 2008'' |
Revision as of 20:31, 30 March 2008
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| , resolution 2.500Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE PHAGE 434 OR2/R1-69 COMPLEX AT 2.5 ANGSTROMS RESOLUTION
Overview
The crystal structure of the DNA-binding domain of bacteriophage 434 repressor (R1-69) in complex with a 20 base-pair DNA fragment has been determined to 2.5 A resolution. The DNA fragment contains the sequence of the OR2 operator site, which differs from the previously studied OR1 site at three of the variable six central base-pairs. Comparison of the two structures shows that the overall bent conformation of the DNA backbone as well as the pattern of DNA-protein interactions seen in the OR1/R1-69 complex are maintained in the OR2 complex. However, the conformations of the DNA base-pairs are different in the two structures. In particular, the central base-pairs of OR2/R1-69 structure are more co-planar than in OR1/R1-69, and there are no cross-strand "bifurcated" hydrogen bonds. These results show that binding of the protein causes operator DNA to adopt a particular, well-defined backbone conformation, and they reinforce the notion that the energetic cost of achieving this conformation, most likely different for different sequences, can determine, at least in part, the relative affinity of the repressor for different operator sites.
About this Structure
1RPE is a Single protein structure of sequence from Phage 434. Full crystallographic information is available from OCA.
Reference
The phage 434 OR2/R1-69 complex at 2.5 A resolution., Shimon LJ, Harrison SC, J Mol Biol. 1993 Aug 5;232(3):826-38. PMID:8355273
Page seeded by OCA on Sun Mar 30 23:31:13 2008
