1rsi
From Proteopedia
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|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=977:2-AMINO-5-BROMO-6-PHENYLPYRIMIDIN-4-OL'>977</scene> | |LIGAND= <scene name='pdbligand=977:2-AMINO-5-BROMO-6-PHENYLPYRIMIDIN-4-OL'>977</scene> | ||
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Dihydroneopterin_aldolase Dihydroneopterin aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.25 4.1.2.25] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydroneopterin_aldolase Dihydroneopterin aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.25 4.1.2.25] </span> |
|GENE= FOLB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus]) | |GENE= FOLB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1rr5|1RR5]], [[1rri|1RRI]], [[1rrw|1RRW]], [[1rry|1RRY]], [[1rs2|1RS2]], [[1rs4|1RS4]], [[1rsd|1RSD]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rsi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rsi OCA], [http://www.ebi.ac.uk/pdbsum/1rsi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rsi RCSB]</span> | ||
}} | }} | ||
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[[Category: Wagner, R.]] | [[Category: Wagner, R.]] | ||
[[Category: Walter, K A.]] | [[Category: Walter, K A.]] | ||
| - | [[Category: 977]] | ||
[[Category: 2-amino-5-bromo-3-hydroxy-6-phenylpyrimidine]] | [[Category: 2-amino-5-bromo-3-hydroxy-6-phenylpyrimidine]] | ||
| - | [[Category: 7 | + | [[Category: 7,8-dihydroneopterin aldolase]] |
| - | + | ||
[[Category: dhna]] | [[Category: dhna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:32:20 2008'' |
Revision as of 20:32, 30 March 2008
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| , resolution 2.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | FOLB (Staphylococcus aureus) | ||||||
| Activity: | Dihydroneopterin aldolase, with EC number 4.1.2.25 | ||||||
| Related: | 1RR5, 1RRI, 1RRW, 1RRY, 1RS2, 1RS4, 1RSD
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
DHNA complex with 2-Amino-5-bromo-3-hydroxy-6-phenylpyrimidine
Overview
Potent inhibitors of 7,8-dihydroneopterin aldolase (DHNA; EC 4.1.2.25) have been discovered using CrystaLEAD X-ray crystallographic high-throughput screening followed by structure-directed optimization. Screening of a 10 000 compound random library provided several low affinity leads and their corresponding X-ray crystal structures bound to the enzyme. The presence of a common structural feature in each of the leads suggested a strategy for the construction of a directed library of approximately 1000 compounds that were screened for inhibitory activity in a traditional enzyme assay. Several lead compounds with IC(50) values of about 1 microM against DHNA were identified, and crystal structures of their enzyme-bound complexes were obtained by cocrystallization. Structure-directed optimization of one of the leads thus identified afforded potent inhibitors with submicromolar IC(50) values.
About this Structure
1RSI is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
Reference
Discovery of potent inhibitors of dihydroneopterin aldolase using CrystaLEAD high-throughput X-ray crystallographic screening and structure-directed lead optimization., Sanders WJ, Nienaber VL, Lerner CG, McCall JO, Merrick SM, Swanson SJ, Harlan JE, Stoll VS, Stamper GF, Betz SF, Condroski KR, Meadows RP, Severin JM, Walter KA, Magdalinos P, Jakob CG, Wagner R, Beutel BA, J Med Chem. 2004 Mar 25;47(7):1709-18. PMID:15027862
Page seeded by OCA on Sun Mar 30 23:32:20 2008
Categories: Dihydroneopterin aldolase | Single protein | Staphylococcus aureus | Betz, S F. | Beutel, B A. | Condroski, K R. | Harlan, J E. | Jakob, C G. | Lerner, C G. | Magdalinos, P. | McCall, J O. | Meadows, R P. | Merrick, S M. | Nienaber, V L. | Sanders, W J. | Severin, J M. | Stamper, G F. | Stoll, V S. | Swanson, S J. | Wagner, R. | Walter, K A. | 2-amino-5-bromo-3-hydroxy-6-phenylpyrimidine | 7,8-dihydroneopterin aldolase | Dhna
