1rsm
From Proteopedia
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|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=NIN:DINITROPHENYLENE'>NIN</scene> | |LIGAND= <scene name='pdbligand=NIN:DINITROPHENYLENE'>NIN</scene> | ||
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rsm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rsm OCA], [http://www.ebi.ac.uk/pdbsum/1rsm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rsm RCSB]</span> | ||
}} | }} | ||
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[[Category: Sheriff, S.]] | [[Category: Sheriff, S.]] | ||
[[Category: Weber, P C.]] | [[Category: Weber, P C.]] | ||
| - | + | [[Category: hydrolase (nucleic acid,rna)]] | |
| - | [[Category: hydrolase (nucleic acid | + | |
| - | + | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:32:22 2008'' |
Revision as of 20:32, 30 March 2008
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| , resolution 2.0Å | |||||||
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| Ligands: | |||||||
| Activity: | Pancreatic ribonuclease, with EC number 3.1.27.5 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE 2-ANGSTROMS RESOLUTION STRUCTURE OF A THERMOSTABLE RIBONUCLEASE A CHEMICALLY CROSS-LINKED BETWEEN LYSINE RESIDUES 7 AND 41
Overview
The crystal structure of Lys-7-(dinitrophenylene)-Lys-41-cross-linked ribonuclease A has been determined by molecular replacement and refined by restrained least-squares methods to an R factor of 0.18 at 2.0-A resolution. Diffraction intensity data were collected by using a conventional diffractometer and an x-ray area detector. Comparison of the thermostable cross-linked protein and the native enzyme shows them to be structurally similar, with a rms difference in backbone and side-chain atoms of 0.52 and 1.34 A, respectively. Native and modified proteins additionally show 35 common bound solvent sites and similar overall temperature factor behavior, despite localized differences resulting from cross-link introduction, altered crystal pH, or lattice interactions with neighboring molecules. These results are discussed in the context of proposals on the origins of thermostability in the cross-linked enzyme.
About this Structure
1RSM is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
The 2-A resolution structure of a thermostable ribonuclease A chemically cross-linked between lysine residues 7 and 41., Weber PC, Sheriff S, Ohlendorf DH, Finzel BC, Salemme FR, Proc Natl Acad Sci U S A. 1985 Dec;82(24):8473-7. PMID:3936036
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