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4uwu

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Revision as of 01:22, 19 January 2017

Lysozyme soaked with a ruthenium based CORM with a pyridine ligand (complex 7)

Structural highlights

4uwu is a 1 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , , ,
Related:	4uwn, 4uwv
Activity:	Lysozyme, with EC number 3.2.1.17
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.^[1]

Publication Abstract from PubMed

A few ruthenium based metal carbonyl complexes, e.g. CORM-2 and CORM-3, have therapeutic activity attributed to their ability to deliver CO to biological targets. In this work, a series of related complexes with the formula [Ru(CO)3Cl2L] (L = DMSO (), l-H3CSO(CH2)2CH(NH2)CO2H) (); d,l-H3CSO(CH2)2CH(NH2)CO2H (); 3-NC5H4(CH2)2SO3Na (); 4-NC5H4(CH2)2SO3Na (); PTA (); DAPTA (); H3CS(CH2)2CH(OH)CO2H (); CNCMe2CO2Me (); CNCMeEtCO2Me (); CN(c-C3H4)CO2Et) ()) were designed, synthesized and studied. The effects of L on their stability, CO release profile, cytotoxicity and anti-inflammatory properties are described. The stability in aqueous solution depends on the nature of L as shown using HPLC and LC-MS studies. The isocyanide derivatives are the least stable complexes, and the S-bound methionine oxide derivative is the more stable one. The complexes do not release CO gas to the headspace, but release CO2 instead. X-ray diffraction of crystals of the model protein Hen Egg White Lysozyme soaked with () and () shows the addition of RuII(CO)(H2O)4 at the His15 binding site. Soakings with () produced the metallacarboxylate [Ru(COOH)(CO)(H2O)3]+ bound to the His15 site. The aqueous chemistry of these complexes is governed by the water-gas shift reaction initiated with the nucleophilic attack of HO- on coordinated CO. DFT calculations show this addition to be essentially barrierless. The complexes have low cytotoxicity and low hemolytic indices. Following i.v. administration of CORM-3, the in vivo bio-distribution of CO differs from that obtained with CO inhalation or with heme oxygenase stimulation. A mechanism for CO transport and delivery from these complexes is proposed.

A contribution to the rational design of Ru(CO)ClL complexes for in vivo delivery of CO.,Seixas JD, Santos MF, Mukhopadhyay A, Coelho AC, Reis PM, Veiros LF, Marques AR, Penacho N, Goncalves AM, Romao MJ, Bernardes GJ, Santos-Silva T, Romao CC Dalton Trans. 2014 Nov 27. PMID:25427784^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Maehashi K, Matano M, Irisawa T, Uchino M, Kashiwagi Y, Watanabe T. Molecular characterization of goose- and chicken-type lysozymes in emu (Dromaius novaehollandiae): evidence for extremely low lysozyme levels in emu egg white. Gene. 2012 Jan 15;492(1):244-9. doi: 10.1016/j.gene.2011.10.021. Epub 2011 Oct, 25. PMID:22044478 doi:10.1016/j.gene.2011.10.021
↑ Seixas JD, Santos MF, Mukhopadhyay A, Coelho AC, Reis PM, Veiros LF, Marques AR, Penacho N, Goncalves AM, Romao MJ, Bernardes GJ, Santos-Silva T, Romao CC. A contribution to the rational design of Ru(CO)ClL complexes for in vivo delivery of CO. Dalton Trans. 2014 Nov 27. PMID:25427784 doi:http://dx.doi.org/10.1039/c4dt02966f

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4uwu"

@@ Line 1: / Line 1: @@
 ==Lysozyme soaked with a ruthenium based CORM with a pyridine ligand (complex 7)==
 <StructureSection load='4uwu' size='340' side='right' caption='[[4uwu]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4uwn|4uwn]], [[4uwv|4uwv]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uwu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uwu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4uwu RCSB], [http://www.ebi.ac.uk/pdbsum/4uwu PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uwu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uwu OCA], [http://pdbe.org/4uwu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4uwu RCSB], [http://www.ebi.ac.uk/pdbsum/4uwu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4uwu ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4uwu" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4uwu

From Proteopedia

Revision as of 01:22, 19 January 2017

Lysozyme soaked with a ruthenium based CORM with a pyridine ligand (complex 7)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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