5h12
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Deep Vent DNA Polymerase== | |
| + | <StructureSection load='5h12' size='340' side='right' caption='[[5h12]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5h12]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H12 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5H12 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5h12 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h12 OCA], [http://pdbe.org/5h12 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h12 RCSB], [http://www.ebi.ac.uk/pdbsum/5h12 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h12 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | DNA polymerases are useful tools in various biochemical experiments. We have focused on the DNA polymerases involved in DNA replication including the unnatural base pair between 7-(2-thienyl)imidazo[4,5-b]pyridine (Ds) and 2-nitro-4-propynylpyrrole (Px). Many reports have described the different combinations between unnatural base pairs and DNA polymerases. As an example, for the replication of the Ds-Px pair, Deep Vent DNA polymerase exhibits high efficiency and fidelity, but Taq DNA polymerase shows much lower efficiency and fidelity. In the present study, we determined the crystal structure of Deep Vent DNA polymerase in the apo form at 2.5 A resolution. Using this structure, we constructed structural models of Deep Vent DNA polymerase complexes with DNA containing an unnatural or natural base in the replication position. The models revealed that the unnatural Ds base in the template-strand DNA clashes with the side-chain oxygen of Thr664 in Taq DNA polymerase, but not in Deep Vent DNA polymerase. | ||
| - | + | Crystal structure of Deep Vent DNA polymerase.,Hikida Y, Kimoto M, Hirao I, Yokoyama S Biochem Biophys Res Commun. 2017 Jan 29;483(1):52-57. doi:, 10.1016/j.bbrc.2017.01.007. Epub 2017 Jan 4. PMID:28063932<ref>PMID:28063932</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5h12" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Hikida, Y]] | ||
| + | [[Category: Hirao, I]] | ||
| + | [[Category: Kimoto, M]] | ||
| + | [[Category: Yokoyama, S]] | ||
| + | [[Category: Dna polymerase]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 23:24, 25 January 2017
Crystal structure of Deep Vent DNA Polymerase
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