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4x2z

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Revision as of 00:18, 26 January 2017

Structural view and substrate specificity of papain-like protease from Avian Infectious Bronchitis Virus

Structural highlights

4x2z is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
NonStd Res:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[R1AB_IBVB] The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.^[1] The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. Activity of PL-PRO is dependent on zinc (By similarity).^[2] The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK (By similarity).^[3] The peptide p16 might be involved in the EGF signaling pathway.^[4] The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) (By similarity).^[5] The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction (By similarity).^[6] Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity).^[7] Nsp9 is a ssRNA-binding protein (By similarity).^[8] NendoU is a Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond (By similarity).^[9]

Publication Abstract from PubMed

Papain-like protease (PLpro) of coronaviruses (CoVs) carries out proteolytic maturation of non-structural proteins that play a role in replication of the virus and performs deubiquitination of host cell factors to scuttle antiviral responses. Avian infectious bronchitis virus (IBV), the causative agent of bronchitis in chicken that results in huge economic losses every year in the poultry industry globally, encodes a PLpro. The substrate specificities of this PLpro are not clearly understood. Here, we show that IBV PLpro can degrade Lys(48)- and Lys(63)-linked polyubiquitin chains to monoubiquitin but not linear polyubiquitin. To explain the substrate specificities, we have solved the crystal structure of PLpro from IBV at 2.15-A resolution. The overall structure is reminiscent of the structure of severe acute respiratory syndrome CoV PLpro. However, unlike the severe acute respiratory syndrome CoV PLpro that lacks blocking loop (BL) 1 of deubiquitinating enzymes, the IBV PLpro has a short BL1-like loop. Access to a conserved catalytic triad consisting of Cys(101), His(264), and Asp(275) is regulated by the flexible BL2. A model of ubiquitin-bound IBV CoV PLpro brings out key differences in substrate binding sites of PLpros. In particular, P3 and P4 subsites as well as residues interacting with the beta-barrel of ubiquitin are different, suggesting different catalytic efficiencies and substrate specificities. We show that IBV PLpro cleaves peptide substrates KKAG-7-amino-4-methylcoumarin and LRGG-7-amino-4-methylcoumarin with different catalytic efficiencies. These results demonstrate that substrate specificities of IBV PLpro are different from other PLpros and that IBV PLpro might target different ubiquitinated host factors to aid the propagation of the virus.

Structural View and Substrate Specificity of Papain-like Protease from Avian Infectious Bronchitis Virus.,Kong L, Shaw N, Yan L, Lou Z, Rao Z J Biol Chem. 2015 Mar 13;290(11):7160-8. doi: 10.1074/jbc.M114.628636. Epub 2015 , Jan 21. PMID:25609249^[10]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Liu C, Xu HY, Liu DX. Induction of caspase-dependent apoptosis in cultured cells by the avian coronavirus infectious bronchitis virus. J Virol. 2001 Jul;75(14):6402-9. PMID:11413307 doi:http://dx.doi.org/10.1128/JVI.75.14.6402-6409.2001
↑ Liu C, Xu HY, Liu DX. Induction of caspase-dependent apoptosis in cultured cells by the avian coronavirus infectious bronchitis virus. J Virol. 2001 Jul;75(14):6402-9. PMID:11413307 doi:http://dx.doi.org/10.1128/JVI.75.14.6402-6409.2001
↑ Liu C, Xu HY, Liu DX. Induction of caspase-dependent apoptosis in cultured cells by the avian coronavirus infectious bronchitis virus. J Virol. 2001 Jul;75(14):6402-9. PMID:11413307 doi:http://dx.doi.org/10.1128/JVI.75.14.6402-6409.2001
↑ Liu C, Xu HY, Liu DX. Induction of caspase-dependent apoptosis in cultured cells by the avian coronavirus infectious bronchitis virus. J Virol. 2001 Jul;75(14):6402-9. PMID:11413307 doi:http://dx.doi.org/10.1128/JVI.75.14.6402-6409.2001
↑ Liu C, Xu HY, Liu DX. Induction of caspase-dependent apoptosis in cultured cells by the avian coronavirus infectious bronchitis virus. J Virol. 2001 Jul;75(14):6402-9. PMID:11413307 doi:http://dx.doi.org/10.1128/JVI.75.14.6402-6409.2001
↑ Liu C, Xu HY, Liu DX. Induction of caspase-dependent apoptosis in cultured cells by the avian coronavirus infectious bronchitis virus. J Virol. 2001 Jul;75(14):6402-9. PMID:11413307 doi:http://dx.doi.org/10.1128/JVI.75.14.6402-6409.2001
↑ Liu C, Xu HY, Liu DX. Induction of caspase-dependent apoptosis in cultured cells by the avian coronavirus infectious bronchitis virus. J Virol. 2001 Jul;75(14):6402-9. PMID:11413307 doi:http://dx.doi.org/10.1128/JVI.75.14.6402-6409.2001
↑ Liu C, Xu HY, Liu DX. Induction of caspase-dependent apoptosis in cultured cells by the avian coronavirus infectious bronchitis virus. J Virol. 2001 Jul;75(14):6402-9. PMID:11413307 doi:http://dx.doi.org/10.1128/JVI.75.14.6402-6409.2001
↑ Liu C, Xu HY, Liu DX. Induction of caspase-dependent apoptosis in cultured cells by the avian coronavirus infectious bronchitis virus. J Virol. 2001 Jul;75(14):6402-9. PMID:11413307 doi:http://dx.doi.org/10.1128/JVI.75.14.6402-6409.2001
↑ Kong L, Shaw N, Yan L, Lou Z, Rao Z. Structural View and Substrate Specificity of Papain-like Protease from Avian Infectious Bronchitis Virus. J Biol Chem. 2015 Mar 13;290(11):7160-8. doi: 10.1074/jbc.M114.628636. Epub 2015 , Jan 21. PMID:25609249 doi:http://dx.doi.org/10.1074/jbc.M114.628636

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4x2z"

@@ Line 1: / Line 1: @@
 ==Structural view and substrate specificity of papain-like protease from Avian Infectious Bronchitis Virus==
 <StructureSection load='4x2z' size='340' side='right' caption='[[4x2z]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x2z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4x2z RCSB], [http://www.ebi.ac.uk/pdbsum/4x2z PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x2z OCA], [http://pdbe.org/4x2z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4x2z RCSB], [http://www.ebi.ac.uk/pdbsum/4x2z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4x2z ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4x2z" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Nonstructural protein|Nonstructural protein]]
 == References ==
 <references/>

4x2z

From Proteopedia

Revision as of 00:18, 26 January 2017

Structural view and substrate specificity of papain-like protease from Avian Infectious Bronchitis Virus

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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