5wqw
From Proteopedia
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wqw OCA], [http://pdbe.org/5wqw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wqw RCSB], [http://www.ebi.ac.uk/pdbsum/5wqw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wqw ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wqw OCA], [http://pdbe.org/5wqw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wqw RCSB], [http://www.ebi.ac.uk/pdbsum/5wqw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wqw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacterial autolysins can partially hydrolyze cell wall peptidoglycans into small sections to regulate cell separation/division and the growth phase. Clostridium perfringens autolysin (Acp) has an N-terminal cell wall-binding domain and a C-terminal catalytic domain with glucosaminidase activity that belongs to the glycoside hydrolase 73 family. Here, we determined the X-ray structure of the Acp catalytic domain (AcpCD) at 1.76 A resolution. AcpCD has a unique crescent-shaped structure, forming a deep groove for substrate-binding at the center of the protein. The modeling study of the enzyme/substrate complex demonstrated that the length of the substrate-binding groove is closely related to the glucosaminidase activity. Mutagenesis analysis showed that AcpCD likely adopts a neighboring-group mechanism for the catalytic reaction. | ||
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| + | Structural and biochemical characterization of the Clostridium perfringens autolysin catalytic domain.,Tamai E, Sekiya H, Goda E, Makihata N, Maki J, Yoshida H, Kamitori S FEBS Lett. 2017 Jan;591(1):231-239. doi: 10.1002/1873-3468.12515. Epub 2016 Dec, 19. PMID:27926788<ref>PMID:27926788</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5wqw" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 11:04, 1 February 2017
X-ray structure of catalytic domain of autolysin from Clostridium perfringens
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