1s7k
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= RimL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 Salmonella typhimurium LT2]) | |GENE= RimL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 Salmonella typhimurium LT2]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1s7f|1S7F]], [[1s7l|1S7L]], [[1s7n|1S7N]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s7k OCA], [http://www.ebi.ac.uk/pdbsum/1s7k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s7k RCSB]</span> | ||
}} | }} | ||
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[[Category: l7/l12]] | [[Category: l7/l12]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:38:32 2008'' |
Revision as of 20:38, 30 March 2008
| |||||||
| , resolution 1.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | RimL (Salmonella typhimurium LT2) | ||||||
| Related: | 1S7F, 1S7L, 1S7N
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase crystal form 2 (apo)
Overview
RimL is responsible for converting the prokaryotic ribosomal protein from L12 to L7 by acetylation of its N-terminal amino group. We demonstrate that purified RimL is capable of posttranslationally acetylating L12, exhibiting a V(max) of 21 min(-1). We have also determined the apostructure of RimL from Salmonella typhimurium and its complex with coenzyme A, revealing a homodimeric oligomer with structural similarity to other Gcn5-related N-acetyltransferase superfamily members. A large central trough located at the dimer interface provides sufficient room to bind both L12 N-terminal helices. Structural and biochemical analysis indicates that RimL proceeds by single-step transfer rather than a covalent-enzyme intermediate. This is the first structure of a Gcn5-related N-acetyltransferase family member with demonstrated activity toward a protein N(alpha)-amino group and is a first step toward understanding the molecular basis for N(alpha)acetylation and its function in cellular regulation.
About this Structure
1S7K is a Single protein structure of sequence from Salmonella typhimurium lt2. Full crystallographic information is available from OCA.
Reference
A novel dimeric structure of the RimL Nalpha-acetyltransferase from Salmonella typhimurium., Vetting MW, de Carvalho LP, Roderick SL, Blanchard JS, J Biol Chem. 2005 Jun 10;280(23):22108-14. Epub 2005 Apr 6. PMID:15817456
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