This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5g5e
From Proteopedia
(Difference between revisions)
m (Protected "5g5e" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystallographic structure of the Tau class glutathione S-transferase MiGSTU from mango Mangifera indica L.== | |
| + | <StructureSection load='5g5e' size='340' side='right' caption='[[5g5e]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5g5e]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G5E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G5E FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5g5f|5g5f]]</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g5e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g5e OCA], [http://pdbe.org/5g5e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g5e RCSB], [http://www.ebi.ac.uk/pdbsum/5g5e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g5e ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We studied a mango glutathione S-transferase (GST) (Mangifera indica) bound to glutathione (GSH) and S-hexyl glutathione (GSX). This GST Tau class (MiGSTU) had a molecular mass of 25.5 kDa. MiGSTU Michaelis-Menten kinetic constants were determined for their substrates obtaining a Km, Vmax and kcat for CDNB of 0.792 mM, 80.58 mM min-1 and 68.49 s-1 respectively and 0.693 mM, 105.32 mM min-1 and 89.57 s-1, for reduced GSH respectively. MiGSTU had a micromolar affinity towards GSH (5.2 muM) or GSX (7.8 muM). The crystal structure of the MiGSTU in apo or bound to GSH or GSX generated a model that explains the thermodynamic signatures of binding and showed the importance of enthalpic-entropic compensation in ligand binding to Tau-class GST enzymes. | ||
| - | + | Insights into ligand binding to a glutathione S-transferase from mango: Structure, thermodynamics and kinetics.,Valenzuela-Chavira I, Contreras-Vergara CA, Arvizu-Flores AA, Serrano-Posada H, Lopez-Zavala AA, Garcia-Orozco KD, Hernandez-Paredes J, Rudino-Pinera E, Stojanoff V, Sotelo-Mundo RR, Islas-Osuna MA Biochimie. 2017 Jan 16. pii: S0300-9084(16)30283-8. doi:, 10.1016/j.biochi.2017.01.005. PMID:28104507<ref>PMID:28104507</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5g5e" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Glutathione transferase]] | ||
[[Category: Hernandez-Paredes, J]] | [[Category: Hernandez-Paredes, J]] | ||
| + | [[Category: Lopez-Zavala, A]] | ||
[[Category: Serrano-Posada, H]] | [[Category: Serrano-Posada, H]] | ||
| + | [[Category: Sotelo-Mundo, R]] | ||
| + | [[Category: Valenzuela-Chavira, I]] | ||
| + | [[Category: Detoxification]] | ||
| + | [[Category: Glutathione s-transferase]] | ||
| + | [[Category: Mango]] | ||
| + | [[Category: Tau class]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 17:51, 1 February 2017
Crystallographic structure of the Tau class glutathione S-transferase MiGSTU from mango Mangifera indica L.
| |||||||||||
