Rho GTPase

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<StructureSection load='1tx4' size='340' side='right' caption='Caption for this structure' scene=''>
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<StructureSection load='1tx4' size='340' side='right' caption='Human RhoA (green) complex with RhoGAP (grey), GDP, AlF4 and Mg+2 ion (green) (PDB code [[1tx4]])' scene=''>
== Function ==
== Function ==
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'''Rho GTPase''' of higher vertebrates include '''RhoA, RhoB''' and '''RhoC'''. These 3 proteins share 85% sequence identity. RhoA is the more extensively studied among these three. RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. A number of proteins have been identified as targets of RhoA<ref>PMID:12642488</ref>.
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'''Rho GTPase''' of higher vertebrates include '''RhoA, RhoB''' and '''RhoC'''. These 3 proteins share 85% sequence identity. RhoA is the more extensively studied among these three. RhoA regulates a signal transduction phosphorylation pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Rho GTPase operates as a switch cycling between the active GTP-bound form and the inactive GDP-bound one. A number of proteins have been identified as targets of RhoA<ref>PMID:12642488</ref>.
== Disease ==
== Disease ==
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== Structural highlights ==
== Structural highlights ==
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The transition state complex of RhoA and RhoGAP contains GDP, AlF4 and Mg+2 ion<ref>PMID:9338791</ref>.
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Revision as of 10:36, 8 February 2017

Human RhoA (green) complex with RhoGAP (grey), GDP, AlF4 and Mg+2 ion (green) (PDB code 1tx4)

Drag the structure with the mouse to rotate

3D structures of Rho GTPase

Updated on 08-February-2017

References

  1. Sepp KJ, Auld VJ. RhoA and Rac1 GTPases mediate the dynamic rearrangement of actin in peripheral glia. Development. 2003 May;130(9):1825-35. PMID:12642488
  2. Zhou J, Hayakawa Y, Wang TC, Bass AJ. RhoA mutations identified in diffuse gastric cancer. Cancer Cell. 2014 Jul 14;26(1):9-11. doi: 10.1016/j.ccr.2014.06.022. PMID:25026207 doi:http://dx.doi.org/10.1016/j.ccr.2014.06.022
  3. Jin L, Burnett AL. RhoA/Rho-kinase in erectile tissue: mechanisms of disease and therapeutic insights. Clin Sci (Lond). 2006 Feb;110(2):153-65. PMID:16411892 doi:http://dx.doi.org/10.1042/CS20050255
  4. Rittinger K, Walker PA, Eccleston JF, Smerdon SJ, Gamblin SJ. Structure at 1.65 A of RhoA and its GTPase-activating protein in complex with a transition-state analogue. Nature. 1997 Oct 16;389(6652):758-62. PMID:9338791 doi:10.1038/39651

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Michal Harel, Alexander Berchansky, Joel L. Sussman

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