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==DNA Polymerase I== | ==DNA Polymerase I== | ||
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Its function is parallel to the eukaryotic DNA polymerase '''BLANK'''. | Its function is parallel to the eukaryotic DNA polymerase '''BLANK'''. | ||
| - | <scene name='75/751160/Dna/1'>DNA</scene | + | <scene name='75/751160/Dna/1'>DNA</scene> |
==Sources== | ==Sources== | ||
Lehman, I. R. (2003). Discovery of DNA polymerase. Journal of Biological Chemistry, 278(37), 34733-34738. | Lehman, I. R. (2003). Discovery of DNA polymerase. Journal of Biological Chemistry, 278(37), 34733-34738. | ||
Revision as of 21:17, 8 February 2017
DNA Polymerase I
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The discovery of DNA Polymerase I is credited to Arthur Kornberg in 1955. Kornberg and his colleagues isolated RNA pol I from E. coli extracts through a DNA assay. SOURCE This enzyme was the first DNA polymerase discovered, and was therefore given the name DNA Polymerase I. It plays a key role in prokaryotic DNA replication.
Function
Its primary role is removing the RNA primers initially laid down by the RNA polymerase primase and replacing those nucleotides with dNTPs. The initial step of excising RNA nucleotides on the replicated strand is made possible due to its 5' to 3' exonuclease activity.
DNA polymerase I also has polymerase activity and 3' to 5' exonuclease activity. Both of the active sites for these functions are found in the Klenow Fragment. Clip thing
The active site in this function is found in the Klenow Fragment which contains both the polymerase and 3' to 5' exonuclease active sites. SOURCE Proteopedia
Its function is parallel to the eukaryotic DNA polymerase BLANK.
Sources
Lehman, I. R. (2003). Discovery of DNA polymerase. Journal of Biological Chemistry, 278(37), 34733-34738.
