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==Function== | ==Function== | ||
| - | + | DNA Polymerase I has the ability to manipulate DNA in three separate ways. It is similar to DNA Polymerase III, as it has 5' to 3' polymerase activity along with 3' to 5' exonuclease activity. Both of the active sites for these functions are found in the Klenow Fragment. '''Clip thing''' | |
| - | DNA polymerase I also has polymerase activity and 3' to 5' exonuclease activity. Both of the active sites for these functions are found in the Klenow Fragment. '''Clip thing''' | ||
| - | + | Its primary role is removing the RNA primers initially laid down by the RNA polymerase primase and replacing those nucleotides with dNTPs. The initial step of excising RNA nucleotides on the replicated strand is made possible due to its 5' to 3' exonuclease activity. The enzyme will remove then add on DNA nucleotides 5' to 3' and connect these nucleotides by phosphodiester bonds. | |
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Its function is parallel to the eukaryotic DNA polymerase '''BLANK'''. | Its function is parallel to the eukaryotic DNA polymerase '''BLANK'''. | ||
| - | <scene name='75/751160/Dna/1'>DNA</scene> | + | DNA polymerase I will <scene name='75/751160/Dna/1'>DNA</scene> |
==Sources== | ==Sources== | ||
Lehman, I. R. (2003). Discovery of DNA polymerase. Journal of Biological Chemistry, 278(37), 34733-34738. | Lehman, I. R. (2003). Discovery of DNA polymerase. Journal of Biological Chemistry, 278(37), 34733-34738. | ||
Revision as of 21:30, 8 February 2017
DNA Polymerase I
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The discovery of DNA Polymerase I is credited to Arthur Kornberg in 1955. Kornberg and his colleagues isolated RNA pol I from E. coli extracts through a DNA assay. SOURCE This enzyme was the first DNA polymerase discovered, and was therefore given the name DNA Polymerase I. It plays a key role in prokaryotic DNA replication.
Function
DNA Polymerase I has the ability to manipulate DNA in three separate ways. It is similar to DNA Polymerase III, as it has 5' to 3' polymerase activity along with 3' to 5' exonuclease activity. Both of the active sites for these functions are found in the Klenow Fragment. Clip thing
Its primary role is removing the RNA primers initially laid down by the RNA polymerase primase and replacing those nucleotides with dNTPs. The initial step of excising RNA nucleotides on the replicated strand is made possible due to its 5' to 3' exonuclease activity. The enzyme will remove then add on DNA nucleotides 5' to 3' and connect these nucleotides by phosphodiester bonds.
Its function is parallel to the eukaryotic DNA polymerase BLANK.
DNA polymerase I will
Sources
Lehman, I. R. (2003). Discovery of DNA polymerase. Journal of Biological Chemistry, 278(37), 34733-34738.
