Sandbox Reserved 1294

General Function

RNA polymerase III is part of the transcription machinery of eukaryotes. It handles genes encoding for small structured RNAs: tRNA, spliceosomal U6 snRNA and 5S rRNA. RNA polymerase III is the largest eukaryotic RNA polymerase, yet it is the least characterized.

Elongation is the process of adding nucleotides to a growing RNA strand. Together, the polymerase, the template DNA, and the growing mRNA strand form the elongation complex. RNA Polymerase III is composed of mobile elements which move relative to each other; conformational changes result throughout the transcription process as a result of this movement.

This page catalogs subunits of RNA Polymerase III which are relevant to elongation complex transcription.

Specific Interactions

The of the polymerase moves the DNA by bridging helix, which allows transcription to proceed. It promotes translocation of pol III, allowing nucleotides to be added continuously.

RPC2 contributes to catatylic activity in the polymerase, and forms the active center of the polymerase along with the largest subunit. It is suggested that RPC helps to open and close the cleft.

RPC1 forms active center together with RPC2. The two subunits form a bridging helix that crosses the cleft near the active site, which binds to the nascent RNA transcript. The helix acts as a ratchet that moves the enlongating transcript through RNA pol III.

RPC6 recruits pol III to the preinitiation complex, and contributes to an initiation-competent configuration for RNA pol III.

RPC10 is involved in transcription reinitiation and RNA cleavage during termination.

The RPC25/RPC8-RPC17/RPC9 subcomplex binds the transcripts emerging from the exit pore, which facilitates elongation.

The RPC53/RPC4-RPC37/RPC5 subcomplex functions to terminate transcription and reinitiate transcription, by providing a binding site for the terminator.

Origin

RNA Polymerase III is used by all eukaryotes in transcription of DNA to mRNA. This process takes place in the nucleus of the cell. The structure shown on this page was elucidated by electron cryomicroscopy of Saccharomyces cerevisiae (bakers yeast). The structure was taken from a 2015 deposition to PDB by Hoffman et al.