Sandbox Reserved 1265
From Proteopedia
(Difference between revisions)
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==Interactions== | ==Interactions== | ||
| - | The complex assembles around the 5' end of mRNA, and then proceeds to scan along the molecule in the 3' direction. | + | The complex assembles around the 5' end of mRNA, and then proceeds to scan along the molecule in the 3' direction. Upon identifying the Kozak sequence, which has the start codon (AUG) embedded within it, the pre-initiation complex binds and begins to recruit the other protein factors necessary for translation. |
The assembly and operation of Human PIC occurs entirely in the cytosol. | The assembly and operation of Human PIC occurs entirely in the cytosol. | ||
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== Origin == | == Origin == | ||
3j8c originates in ''Homo sapiens'', but similar proteins are found in virtually all eukaryotes. | 3j8c originates in ''Homo sapiens'', but similar proteins are found in virtually all eukaryotes. | ||
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| + | Prokaryotes do not have a corresponding initiation complex - instead, most have some deviation of the Shine-Dalgarno sequence in the 5' untranslated region that the 30s ribosomal subunit binds to in order to initiate translation. | ||
== References == | == References == | ||
Current revision
Human Pre-initiation Complex (3j8c)
Structure
Human Pre-initiation complex is an octameric molecule - it is composed of 8 polypeptide chain subunits.
The 43S initiation complex forms when Methionine initiation tRNA (Met-tRNAi(Met)) forms a ternary complex with Eukaryotic Initiation factor 2 (EIF2) and GTP. This complex then binds to the 40S subunit of the ribosome alongside EIF3, EIF1, and EIF1A. Finally, DHX29, a protein that aids structured mRNA scanning, binds to the 40S subunit as well.
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