1sj7
From Proteopedia
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|PDB= 1sj7 |SIZE=350|CAPTION= <scene name='initialview01'>1sj7</scene>, resolution 2.50Å | |PDB= 1sj7 |SIZE=350|CAPTION= <scene name='initialview01'>1sj7</scene>, resolution 2.50Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=PT:PLATINUM (II) ION'>PT</scene> | + | |LIGAND= <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= TLN1, TLN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | |GENE= TLN1, TLN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1sj8|1SJ8]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sj7 OCA], [http://www.ebi.ac.uk/pdbsum/1sj7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1sj7 RCSB]</span> | ||
}} | }} | ||
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[[Category: Gingras, A R.]] | [[Category: Gingras, A R.]] | ||
[[Category: Papagrigoriou, E.]] | [[Category: Papagrigoriou, E.]] | ||
| - | [[Category: PT]] | ||
[[Category: structural protein]] | [[Category: structural protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:42:47 2008'' |
Revision as of 20:42, 30 March 2008
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| , resolution 2.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | TLN1, TLN (Mus musculus) | ||||||
| Related: | 1SJ8
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Talin Rod 482-655
Overview
The interaction between the cytoskeletal proteins talin and vinculin plays a key role in integrin-mediated cell adhesion and migration. We have determined the crystal structures of two domains from the talin rod spanning residues 482-789. Talin 482-655, which contains a vinculin-binding site (VBS), folds into a five-helix bundle whereas talin 656-789 is a four-helix bundle. We show that the VBS is composed of a hydrophobic surface spanning five turns of helix 4. All the key side chains from the VBS are buried and contribute to the hydrophobic core of the talin 482-655 fold. We demonstrate that the talin 482-655 five-helix bundle represents an inactive conformation, and mutations that disrupt the hydrophobic core or deletion of helix 5 are required to induce an active conformation in which the VBS is exposed. We also report the crystal structure of the N-terminal vinculin head domain in complex with an activated form of talin. Activation of the VBS in talin and the recruitment of vinculin may support the maturation of small integrin/talin complexes into more stable adhesions.
About this Structure
1SJ7 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Activation of a vinculin-binding site in the talin rod involves rearrangement of a five-helix bundle., Papagrigoriou E, Gingras AR, Barsukov IL, Bate N, Fillingham IJ, Patel B, Frank R, Ziegler WH, Roberts GC, Critchley DR, Emsley J, EMBO J. 2004 Aug 4;23(15):2942-51. Epub 2004 Jul 22. PMID:15272303
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