1sra

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|PDB= 1sra |SIZE=350|CAPTION= <scene name='initialview01'>1sra</scene>, resolution 2.0&Aring;
|PDB= 1sra |SIZE=350|CAPTION= <scene name='initialview01'>1sra</scene>, resolution 2.0&Aring;
|SITE=
|SITE=
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|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
|ACTIVITY=
|ACTIVITY=
|GENE= HUMAN BM-40 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
|GENE= HUMAN BM-40 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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|DOMAIN=
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sra FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sra OCA], [http://www.ebi.ac.uk/pdbsum/1sra PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1sra RCSB]</span>
}}
}}
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[[Category: Maurer, P.]]
[[Category: Maurer, P.]]
[[Category: Timpl, R.]]
[[Category: Timpl, R.]]
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[[Category: CA]]
 
[[Category: extracellular matrix protein]]
[[Category: extracellular matrix protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:08:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:45:57 2008''

Revision as of 20:45, 30 March 2008


PDB ID 1sra

Drag the structure with the mouse to rotate
, resolution 2.0Å
Ligands:
Gene: HUMAN BM-40 (Homo sapiens)
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



STRUCTURE OF A NOVEL EXTRACELLULAR CA2+-BINDING MODULE IN BM-40(SLASH)SPARC(SLASH)OSTEONECTIN


Overview

The EF-hand is a highly conserved Ca(2+)-binding motif found in many cytosolic Ca(2+)-modulated proteins. Here we report the crystal structure at 2.0 A resolution of the carboxy-terminal domain of human BM-40 (SPARC, osteonectin), an extracellular matrix protein containing an EF-hand pair. The two EF-hands interact canonically but their detailed structures are unusual. In the first EF-hand a one-residue insertion is accommodated by a cis-peptide bond and by substituting a carboxylate by a peptide carbonyl as a Ca2+ ligand. The second EF-hand is stabilized by a disulphide bond. The EF-hand pair interacts tightly with an amphiphilic amino-terminal helix, reminiscent of target peptide binding by calmodulin. The present structure defines a novel protein module occurring in several other extracellular proteins.

About this Structure

1SRA is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of a novel extracellular Ca(2+)-binding module in BM-40., Hohenester E, Maurer P, Hohenadl C, Timpl R, Jansonius JN, Engel J, Nat Struct Biol. 1996 Jan;3(1):67-73. PMID:8548457

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