1sxh
From Proteopedia
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|PDB= 1sxh |SIZE=350|CAPTION= <scene name='initialview01'>1sxh</scene>, resolution 2.75Å | |PDB= 1sxh |SIZE=350|CAPTION= <scene name='initialview01'>1sxh</scene>, resolution 2.75Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= CCpa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1404 Bacillus megaterium]) | |GENE= CCpa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1404 Bacillus megaterium]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1sxg|1SXG]], [[1sxi|1SXI]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sxh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sxh OCA], [http://www.ebi.ac.uk/pdbsum/1sxh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1sxh RCSB]</span> | ||
}} | }} | ||
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[[Category: Schumacher, M A.]] | [[Category: Schumacher, M A.]] | ||
[[Category: Seidel, G.]] | [[Category: Seidel, G.]] | ||
| - | [[Category: allosterism | + | [[Category: allosterism]] |
| + | [[Category: ccr]] | ||
| + | [[Category: gram positive bacteria]] | ||
| + | [[Category: phosphoprotein]] | ||
| + | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:48:18 2008'' |
Revision as of 20:48, 30 March 2008
| |||||||
| , resolution 2.75Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | CCpa (Bacillus megaterium) | ||||||
| Related: | 1SXG, 1SXI
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
apo structure of B. megaterium transcription regulator
Overview
Carbon catabolite repression (CCR) is one of the most fundamental environmental-sensing mechanisms in bacteria and imparts competitive advantage by establishing priorities in carbon metabolism. In gram-positive bacteria, the master transcription regulator of CCR is CcpA. CcpA is a LacI-GalR family member that employs, as an allosteric corepressor, the phosphoprotein HPr-Ser46-P, which is formed in glucose-replete conditions. Here we report structures of the Bacillus megaterium apoCcpA and a CcpA-(HPr-Ser46-P)-DNA complex. These structures reveal that HPr-Ser46-P mediates a novel two-component allosteric DNA binding activation mechanism that involves both rotation of the CcpA subdomains and relocation of pivot-point residue Thr61, which leads to juxtaposition of the DNA binding regions permitting "hinge" helix formation in the presence of cognate DNA. The structure of the CcpA-(HPr-Ser46-P)-cre complex also reveals the elegant mechanism by which CcpA family-specific interactions with HPr-Ser46-P residues Ser46-P and His15 partition the high-energy CCR and low-energy PTS pathways, the latter requiring HPr-His15-P.
About this Structure
1SXH is a Single protein structure of sequence from Bacillus megaterium. Full crystallographic information is available from OCA.
Reference
Structural basis for allosteric control of the transcription regulator CcpA by the phosphoprotein HPr-Ser46-P., Schumacher MA, Allen GS, Diel M, Seidel G, Hillen W, Brennan RG, Cell. 2004 Sep 17;118(6):731-41. PMID:15369672
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