5guy
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Structural and functional study of chuY from E. coli CFT073 strain== | |
| + | <StructureSection load='5guy' size='340' side='right' caption='[[5guy]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5guy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GUY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GUY FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5guy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5guy OCA], [http://pdbe.org/5guy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5guy RCSB], [http://www.ebi.ac.uk/pdbsum/5guy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5guy ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The uropathogenic Escherichia coli strain CFT073 contains multiple iron and heme transport systems, which facilitate infection of the host urinary tract. To elucidate the molecular and cellular function of ChuY, a hypothetical gene in the heme degradation/utilization pathway, we solved the crystal structure of ChuY at 2.4 A resolution. ChuY has high structural homology with human biliverdin and flavin reductase. We confirmed that ChuY has flavin mononucleotide (FMN) reductase activity, using NAD(P)H as a cofactor, and shows porphyrin ring binding affinity. A chuY deletion-insertion strain showed reduced survival potential compared to wild-type and complemented strains in mammalian cells. Current results suggest ChuY acts as a reductase in heme homeostasis to maintain the virulence potential of E. coli CFT073. | ||
| - | + | Structural and functional study of ChuY from Escherichia coli strain CFT073.,Kim H, Chaurasia AK, Kim T, Choi J, Ha SC, Kim D, Kim KK Biochem Biophys Res Commun. 2017 Jan 22;482(4):1176-1182. doi:, 10.1016/j.bbrc.2016.12.008. Epub 2016 Dec 3. PMID:27919686<ref>PMID:27919686</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5guy" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Chaurasia, A K]] | ||
| + | [[Category: Choi, J]] | ||
| + | [[Category: HA, S C]] | ||
| + | [[Category: Kim, D]] | ||
| + | [[Category: Kim, H]] | ||
| + | [[Category: Kim, K K]] | ||
| + | [[Category: Chuy]] | ||
| + | [[Category: Flavin reductase]] | ||
| + | [[Category: Oxidoreductase]] | ||
Revision as of 16:44, 9 March 2017
Structural and functional study of chuY from E. coli CFT073 strain
| |||||||||||
Categories: Chaurasia, A K | Choi, J | HA, S C | Kim, D | Kim, H | Kim, K K | Chuy | Flavin reductase | Oxidoreductase
