1t6c
From Proteopedia
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|PDB= 1t6c |SIZE=350|CAPTION= <scene name='initialview01'>1t6c</scene>, resolution 1.53Å | |PDB= 1t6c |SIZE=350|CAPTION= <scene name='initialview01'>1t6c</scene>, resolution 1.53Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= ppx ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224324 Aquifex aeolicus VF5]) | |GENE= ppx ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224324 Aquifex aeolicus VF5]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1t6d|1T6D]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t6c OCA], [http://www.ebi.ac.uk/pdbsum/1t6c PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t6c RCSB]</span> | ||
}} | }} | ||
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[[Category: Laurberg, M.]] | [[Category: Laurberg, M.]] | ||
[[Category: Liljas, A.]] | [[Category: Liljas, A.]] | ||
| - | [[Category: CA]] | ||
| - | [[Category: CL]] | ||
| - | [[Category: IOD]] | ||
| - | [[Category: MPD]] | ||
[[Category: actin-like fold]] | [[Category: actin-like fold]] | ||
[[Category: alpha/beta protein]] | [[Category: alpha/beta protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:52:00 2008'' |
Revision as of 20:52, 30 March 2008
| |||||||
| , resolution 1.53Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Gene: | ppx (Aquifex aeolicus VF5) | ||||||
| Related: | 1T6D
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structural characterization of the Ppx/GppA protein family: crystal structure of the Aquifex aeolicus family member
Overview
Exopolyphosphatase/guanosine pentaphosphate phosphohydrolase (PPX/GPPA) enzymes play central roles in the bacterial stringent response induced by starvation. The high-resolution crystal structure of the putative Aquifex aeolicus PPX/GPPA phosphatase from the actin-like ATPase domain superfamily has been determined, providing the first insights to features of the common catalytic core of the PPX/GPPA family. The protein has a two-domain structure with an active site located in the interdomain cleft. Two crystal forms were investigated (type I and II) at resolutions of 1.53 and 2.15 A, respectively. This revealed a structural flexibility that has previously been described as a "butterfly-like" cleft opening around the active site in other actin-like superfamily proteins. A calcium ion is observed at the center of this region in type I crystals, substantiating that PPX/GPPA enzymes use metal ions for catalysis. Structural analysis suggests that nucleotides bind at a similar position to that seen in other members of the superfamily.
About this Structure
1T6C is a Single protein structure of sequence from Aquifex aeolicus vf5. Full crystallographic information is available from OCA.
Reference
Structural characterization of the stringent response related exopolyphosphatase/guanosine pentaphosphate phosphohydrolase protein family., Kristensen O, Laurberg M, Liljas A, Kastrup JS, Gajhede M, Biochemistry. 2004 Jul 20;43(28):8894-900. PMID:15248747
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