1t7p
From Proteopedia
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|PDB= 1t7p |SIZE=350|CAPTION= <scene name='initialview01'>1t7p</scene>, resolution 2.2Å | |PDB= 1t7p |SIZE=350|CAPTION= <scene name='initialview01'>1t7p</scene>, resolution 2.2Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=2DA:2',3'-DIDEOXYADENOSINE-5'-MONOPHOSPHATE'>2DA</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DG3:2'-3'-DIDEOXYGUANOSINE-5'-TRIPHOSPHATE'>DG3</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t7p OCA], [http://www.ebi.ac.uk/pdbsum/1t7p PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t7p RCSB]</span> | ||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1T7P is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ | + | 1T7P is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T7P OCA]. |
==Reference== | ==Reference== | ||
Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 A resolution., Doublie S, Tabor S, Long AM, Richardson CC, Ellenberger T, Nature. 1998 Jan 15;391(6664):251-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9440688 9440688] | Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 A resolution., Doublie S, Tabor S, Long AM, Richardson CC, Ellenberger T, Nature. 1998 Jan 15;391(6664):251-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9440688 9440688] | ||
| - | [[Category: Bacteriophage t7]] | ||
[[Category: DNA-directed DNA polymerase]] | [[Category: DNA-directed DNA polymerase]] | ||
| + | [[Category: Enterobacteria phage t7]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: Richardson, C C.]] | [[Category: Richardson, C C.]] | ||
[[Category: Tabor, S.]] | [[Category: Tabor, S.]] | ||
| - | [[Category: DG3]] | ||
| - | [[Category: MG]] | ||
[[Category: complex (hydrolase/electron transport/dna)]] | [[Category: complex (hydrolase/electron transport/dna)]] | ||
[[Category: dna replication]] | [[Category: dna replication]] | ||
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[[Category: thioredoxin]] | [[Category: thioredoxin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:52:26 2008'' |
Revision as of 20:52, 30 March 2008
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| , resolution 2.2Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , , | ||||||
| Activity: | DNA-directed DNA polymerase, with EC number 2.7.7.7 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
T7 DNA POLYMERASE COMPLEXED TO DNA PRIMER/TEMPLATE,A NUCLEOSIDE TRIPHOSPHATE, AND ITS PROCESSIVITY FACTOR THIOREDOXIN
Overview
DNA polymerases change their specificity for nucleotide substrates with each catalytic cycle, while achieving error frequencies in the range of 10(-5) to 10(-6). Here we present a 2.2 A crystal structure of the replicative DNA polymerase from bacteriophage T7 complexed with a primer-template and a nucleoside triphosphate in the polymerase active site. The structure illustrates how nucleotides are selected in a template-directed manner, and provides a structural basis for a metal-assisted mechanism of phosphoryl transfer by a large group of related polymerases.
About this Structure
1T7P is a Protein complex structure of sequences from Enterobacteria phage t7 and Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 A resolution., Doublie S, Tabor S, Long AM, Richardson CC, Ellenberger T, Nature. 1998 Jan 15;391(6664):251-8. PMID:9440688
Page seeded by OCA on Sun Mar 30 23:52:26 2008
Categories: DNA-directed DNA polymerase | Enterobacteria phage t7 | Escherichia coli | Protein complex | Doublie, S. | Ellenberger, T. | Long, A M. | Richardson, C C. | Tabor, S. | Complex (hydrolase/electron transport/dna) | Dna replication | Nucleotidyl transferase | Processivity factor | Sequencing | T7 dna polymerase | Thioredoxin
