5g2r
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the Mo-insertase domain Cnx1E from Arabidopsis thaliana== | |
| + | <StructureSection load='5g2r' size='340' side='right' caption='[[5g2r]], [[Resolution|resolution]] 2.45Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5g2r]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G2R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G2R FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5g2s|5g2s]]</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Molybdopterin_molybdotransferase Molybdopterin molybdotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.10.1.1 2.10.1.1] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g2r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g2r OCA], [http://pdbe.org/5g2r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g2r RCSB], [http://www.ebi.ac.uk/pdbsum/5g2r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g2r ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/CNX1_ARATH CNX1_ARATH]] Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.<ref>PMID:15504727</ref> <ref>PMID:16636046</ref> <ref>PMID:12590921</ref> <ref>PMID:15306815</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The molybdenum cofactor (Moco) is a redox active prosthetic group, essentially required for numerous enzyme-catalyzed two electron transfer reactions. Moco is synthesized by an evolutionarily old and highly conserved multistep pathway. In the last step of Moco biosynthesis, the molybdenum center is inserted into the final Moco precursor adenylated molybdopterin (MPT-AMP). This unique and yet poorly characterized maturation reaction finally yields physiologically active Moco. In the model plant Arabidopsis, the two domain enzyme, Cnx1, is required for Moco formation. Recently, a genetic screen identified novel Arabidopsis cnx1 mutant plant lines each harboring a single amino acid exchange in the N-terminal Cnx1E domain. Biochemical characterization of the respective recombinant Cnx1E variants revealed two different amino acid exchanges (S197F and G175D) that impair Cnx1E dimerization, thus linking Cnx1E oligomerization to Cnx1 functionality. Analysis of the Cnx1E structure identified Cnx1E active site-bound molybdate and magnesium ions, which allowed to fine-map the Cnx1E MPT-AMP-binding site. | ||
| - | + | Dimerization of the plant molybdenum insertase Cnx1E is required for synthesis of the molybdenum cofactor.,Krausze J, Probst C, Curth U, Reichelt J, Saha S, Schafflick D, Heinz DW, Mendel RR, Kruse T Biochem J. 2017 Jan 1;474(1):163-178. doi: 10.1042/BCJ20160846. Epub 2016 Nov 1. PMID:27803248<ref>PMID:27803248</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5g2r" style="background-color:#fffaf0;"></div> | |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Molybdopterin molybdotransferase]] | ||
| + | [[Category: Heinz, D W]] | ||
[[Category: Krausze, J]] | [[Category: Krausze, J]] | ||
| - | [[Category: Heinz, D.W]] | ||
[[Category: Kruse, T]] | [[Category: Kruse, T]] | ||
| + | [[Category: Mendel, R R]] | ||
| + | [[Category: Probst, C]] | ||
| + | [[Category: Saha, S]] | ||
| + | [[Category: Cnx1]] | ||
| + | [[Category: Cnx1e]] | ||
| + | [[Category: Gephe]] | ||
| + | [[Category: Gephyrin]] | ||
| + | [[Category: Mo-insertase]] | ||
| + | [[Category: Moco]] | ||
| + | [[Category: Moea]] | ||
| + | [[Category: Molybdenum cofactor]] | ||
| + | [[Category: Molybdenum metabolism]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 17:35, 10 March 2017
Crystal structure of the Mo-insertase domain Cnx1E from Arabidopsis thaliana
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Categories: Molybdopterin molybdotransferase | Heinz, D W | Krausze, J | Kruse, T | Mendel, R R | Probst, C | Saha, S | Cnx1 | Cnx1e | Gephe | Gephyrin | Mo-insertase | Moco | Moea | Molybdenum cofactor | Molybdenum metabolism | Transferase
