5gqu
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142== | |
| + | <StructureSection load='5gqu' size='340' side='right' caption='[[5gqu]], [[Resolution|resolution]] 1.85Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5gqu]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GQU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GQU FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5gqv|5gqv]], [[5gqw|5gqw]], [[5gqx|5gqx]], [[5gqy|5gqy]], [[5gqz|5gqz]], [[5gr0|5gr0]], [[5gr1|5gr1]], [[5gr2|5gr2]], [[5gr3|5gr3]], [[5gr4|5gr4]], [[5gr5|5gr5]], [[5gr6|5gr6]]</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,4-alpha-glucan_branching_enzyme 1,4-alpha-glucan branching enzyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.18 2.4.1.18] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gqu OCA], [http://pdbe.org/5gqu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gqu RCSB], [http://www.ebi.ac.uk/pdbsum/5gqu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gqu ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/B1WPM8_CYAA5 B1WPM8_CYAA5]] Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.[HAMAP-Rule:MF_00685] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Several cyanobacterial species, including Cyanothece sp. ATCC 51142, remarkably have four isoforms of alpha-glucan branching enzymes (BEs). Based on their primary structures, they are classified into glycoside hydrolase (GH) family 13 (BE1, BE2 and BE3) or family 57 (GH57 BE). In the present study, GH13-type BEs from Cyanothece sp. ATCC 51142 (BE1, BE2 and BE3) have been overexpressed in Escherichia coli and biochemically characterized. The recombinant BE1 was crystallized by the hanging-drop vapour-diffusion method. Crystals of BE1 were obtained at 293 K in the presence of 0.2 M Mg(2+), 7-10%(w/v) ethanol, 0.1 M HEPES-NaOH pH 7.2-7.9. The crystals belonged to the tetragonal space group P41212, with unit-cell parameters a = b = 133.75, c = 185.90 A, and diffracted to beyond 1.85 A resolution. Matthews coefficient calculations suggested that the crystals of BE1 contained two molecules in the asymmetric unit. | ||
| - | + | Crystallization and crystallographic analysis of branching enzymes from Cyanothece sp. ATCC 51142.,Hayashi M, Suzuki R, Colleoni C, Ball SG, Fujita N, Suzuki E Acta Crystallogr F Struct Biol Commun. 2015 Aug;71(Pt 8):1109-13. doi:, 10.1107/S2053230X1501198X. Epub 2015 Jul 29. PMID:26249708<ref>PMID:26249708</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5gqu" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: 1,4-alpha-glucan branching enzyme]] | ||
| + | [[Category: Suzuki, E]] | ||
| + | [[Category: Suzuki, R]] | ||
| + | [[Category: Branching enzyme]] | ||
| + | [[Category: Cyanobacteria]] | ||
| + | [[Category: Glycoside hydrolase family 13]] | ||
| + | [[Category: Starch]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 01:31, 11 March 2017
Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142
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