4xae

From Proteopedia

(Difference between revisions)

Revision as of 02:03, 11 March 2017

Structure of Feruloyl-CoA 6-hydroxylase (F6H) from Arabidopsis thaliana

Structural highlights

4xae is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
NonStd Res:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[F6H1_ARATH] 2-oxoglutarate (OG)- and Fe(II)-dependent dioxygenase (2OGD)involved in scopoletin biosynthesis. Converts feruloyl CoA into 6'-hydroxyferuloyl CoA but has no activity with ferulic acid, feruloylquinic acid, caffeic acid, caffeoyl CoA, p-coumaric acid, cinnamic acid, cinnamoyl CoA or benzoyl CoA.^[1]

Publication Abstract from PubMed

Coumarins belong to an important class of plant secondary metabolites. Feruloyl-CoA 6'-hydroxylase (F6'H), a 2-oxoglutarate dependent dioxygenase (2OGD), catalyzes a pivotal step in the biosynthesis of a simple coumarin scopoletin. In this study, we determined the 3-dimensional structure of the F6'H1 apo enzyme by X-ray crystallography. It is the first reported structure of a 2OGD enzyme involved in coumarin biosynthesis and closely resembles the structure of Arabidopsis thaliana anthocyanidin synthase. To better understand the mechanism of enzyme catalysis and substrate specificity, we also generated a homology model of a related ortho-hydroxylase (C2'H) from sweet potato. By comparing these two structures, we targeted two amino acid residues and verified their roles in substrate binding and specificity by site-directed mutagenesis.

Structural Insights into Substrate Specificity of Feruloyl-CoA 6'-Hydroxylase from Arabidopsis thaliana.,Sun X, Zhou D, Kandavelu P, Zhang H, Yuan Q, Wang BC, Rose J, Yan Y Sci Rep. 2015 May 20;5:10355. doi: 10.1038/srep10355. PMID:25993561^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kai K, Mizutani M, Kawamura N, Yamamoto R, Tamai M, Yamaguchi H, Sakata K, Shimizu B. Scopoletin is biosynthesized via ortho-hydroxylation of feruloyl CoA by a 2-oxoglutarate-dependent dioxygenase in Arabidopsis thaliana. Plant J. 2008 Sep;55(6):989-99. doi: 10.1111/j.1365-313X.2008.03568.x. Epub 2008 , Jun 10. PMID:18547395 doi:http://dx.doi.org/10.1111/j.1365-313X.2008.03568.x
↑ Sun X, Zhou D, Kandavelu P, Zhang H, Yuan Q, Wang BC, Rose J, Yan Y. Structural Insights into Substrate Specificity of Feruloyl-CoA 6'-Hydroxylase from Arabidopsis thaliana. Sci Rep. 2015 May 20;5:10355. doi: 10.1038/srep10355. PMID:25993561 doi:http://dx.doi.org/10.1038/srep10355

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4xae"

@@ Line 1: / Line 1: @@
 ==Structure of Feruloyl-CoA 6-hydroxylase (F6H) from Arabidopsis thaliana==
 <StructureSection load='4xae' size='340' side='right' caption='[[4xae]], [[Resolution|resolution]] 2.77&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xae OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xae RCSB], [http://www.ebi.ac.uk/pdbsum/4xae PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xae OCA], [http://pdbe.org/4xae PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xae RCSB], [http://www.ebi.ac.uk/pdbsum/4xae PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xae ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4xae" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4xae

From Proteopedia

Revision as of 02:03, 11 March 2017

Structure of Feruloyl-CoA 6-hydroxylase (F6H) from Arabidopsis thaliana

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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