5b3i
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Homo-dimeric structure of cytochrome c' from Thermophilic Hydrogenophilus thermoluteolus== | |
| + | <StructureSection load='5b3i' size='340' side='right' caption='[[5b3i]], [[Resolution|resolution]] 1.89Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5b3i]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Hydrogenophilus_thermoluteolus Hydrogenophilus thermoluteolus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B3I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B3I FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b3i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b3i OCA], [http://pdbe.org/5b3i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b3i RCSB], [http://www.ebi.ac.uk/pdbsum/5b3i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5b3i ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Thermophilic Hydrogenophilus thermoluteolus cytochrome c' (PHCP) exhibits higher thermal stability than a mesophilic counterpart, Allochromatium vinosum cytochrome c' (AVCP), which has a homo-dimeric structure and ligand-binding ability. To understand the thermal stability mechanism and ligand-binding ability of the thermally stable PHCP protein, the crystal structure of PHCP was first determined. It formed a homo-dimeric structure, the main chain root mean square deviation (rmsd) value between PHCP and AVCP being 0.65 A. In the PHCP structure, six specific residues appeared to strengthen the heme-related and subunit-subunit interactions, which were not conserved in the AVCP structure. PHCP variants having altered subunit-subunit interactions were more severely destabilized than ones having altered heme-related interactions. The PHCP structure further revealed a ligand-binding channel and a penta-coordinated heme, as observed in the AVCP protein. A spectroscopic study clearly showed that some ligands were bound to the PHCP protein. It is concluded that the dimeric PHCP from the thermophile is effectively stabilized through heme-related and subunit-subunit interactions with conservation of the ligand-binding ability. BRIEF SUMMARY: We report the X-ray crystal structure of cytochrome c' (PHCP) from thermophilic Hydrogenophilus thermoluteolus. The high thermal stability of PHCP was attributed to heme-related and subunit-subunit interactions, which were confirmed by a mutagenesis study. The ligand-binding ability of PHCP was examined by spectrophotometry. PHCP acquired the thermal stability with conservation of the ligand-binding ability. This study furthers the understanding of the stability and function of cytochromes c. | ||
| - | + | Structural and functional insights into thermally stable cytochrome c' from a thermophile.,Fujii S, Oki H, Kawahara K, Yamane D, Yamanaka M, Maruno T, Kobayashi Y, Masanari M, Wakai S, Nishihara H, Ohkubo T, Sambongi Y Protein Sci. 2017 Jan 18. doi: 10.1002/pro.3120. PMID:28097774<ref>PMID:28097774</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5b3i" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Hydrogenophilus thermoluteolus]] | ||
| + | [[Category: Fujii, S]] | ||
| + | [[Category: Kawahara, K]] | ||
| + | [[Category: Kobayashi, Y]] | ||
| + | [[Category: Maruno, T]] | ||
| + | [[Category: Masanari, M]] | ||
| + | [[Category: Nishihara, H]] | ||
| + | [[Category: Ohkubo, T]] | ||
| + | [[Category: Oki, H]] | ||
| + | [[Category: Sambongi, Y]] | ||
| + | [[Category: Wakai, S]] | ||
| + | [[Category: Yamanaka, M]] | ||
| + | [[Category: Yamane, D]] | ||
| + | [[Category: Class ii cytochrome c protein]] | ||
| + | [[Category: Cytochrome cs']] | ||
| + | [[Category: Electron transport]] | ||
| + | [[Category: Heme protein]] | ||
Revision as of 09:30, 11 March 2017
Homo-dimeric structure of cytochrome c' from Thermophilic Hydrogenophilus thermoluteolus
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