1tez
From Proteopedia
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|PDB= 1tez |SIZE=350|CAPTION= <scene name='initialview01'>1tez</scene>, resolution 1.80Å | |PDB= 1tez |SIZE=350|CAPTION= <scene name='initialview01'>1tez</scene>, resolution 1.80Å | ||
|SITE= <scene name='pdbsite=ACT:Upon+Complexation+w.+The+Cpd-Damage+The+Active+Site+Appa+...'>ACT</scene> | |SITE= <scene name='pdbsite=ACT:Upon+Complexation+w.+The+Cpd-Damage+The+Active+Site+Appa+...'>ACT</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=HDF:8-HYDROXY-10-(D-RIBO-2,3,4,5-TETRAHYDROXYPENTYL)-5-DEAZAISOALLOXAZINE'>HDF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TCP:5'-METHYLTHYMIDINE'>TCP</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Deoxyribodipyrimidine_photo-lyase Deoxyribodipyrimidine photo-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.3 4.1.99.3] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribodipyrimidine_photo-lyase Deoxyribodipyrimidine photo-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.3 4.1.99.3] </span> |
|GENE= PHR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1131 Synechococcus sp.]) | |GENE= PHR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1131 Synechococcus sp.]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tez OCA], [http://www.ebi.ac.uk/pdbsum/1tez PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1tez RCSB]</span> | ||
}} | }} | ||
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[[Category: Klar, T.]] | [[Category: Klar, T.]] | ||
[[Category: Mees, A.]] | [[Category: Mees, A.]] | ||
| - | [[Category: | + | [[Category: dna repair]] |
| - | [[Category: | + | [[Category: light-driven electron transfer]] |
| - | + | [[Category: photolyase]] | |
| - | [[Category: photolyase | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:55:06 2008'' |
Revision as of 20:55, 30 March 2008
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| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , , , , , , , | ||||||
| Gene: | PHR (Synechococcus sp.) | ||||||
| Activity: | Deoxyribodipyrimidine photo-lyase, with EC number 4.1.99.3 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
COMPLEX BETWEEN DNA AND THE DNA PHOTOLYASE FROM ANACYSTIS NIDULANS
Overview
DNA photolyases use light energy to repair DNA that comprises ultraviolet-induced lesions such as the cis-syn cyclobutane pyrimidine dimers (CPDs). Here we report the crystal structure of a DNA photolyase bound to duplex DNA that is bent by 50 degrees and comprises a synthetic CPD lesion. This CPD lesion is flipped into the active site and split there into two thymines by synchrotron radiation at 100 K. Although photolyases catalyze blue light-driven CPD cleavage only above 200 K, this structure apparently mimics a structural substate during light-driven DNA repair in which back-flipping of the thymines into duplex DNA has not yet taken place.
About this Structure
1TEZ is a Single protein structure of sequence from Synechococcus sp.. Full crystallographic information is available from OCA.
Reference
Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair., Mees A, Klar T, Gnau P, Hennecke U, Eker AP, Carell T, Essen LO, Science. 2004 Dec 3;306(5702):1789-93. PMID:15576622
Page seeded by OCA on Sun Mar 30 23:55:06 2008
