2c4b

From Proteopedia

(Difference between revisions)

Revision as of 13:16, 15 March 2017

Inhibitor cystine knot protein McoEeTI fused to the catalytically inactive barnase mutant H102A

Structural highlights

2c4b is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , , , , ,
Activity:	Ribonuclease T(1), with EC number 3.1.27.3
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RNBR_BACAM] Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We present a fusion system suited to determine the crystal structure of small disulfide-rich proteins. McoEeTI, a hybrid inhibitor cystine knot microprotein, was produced as a soluble fusion to a catalytically inactive variant of the RNAse barnase in Escherichia coli. Functioning as a versatile tag, barnase facilitated purification, crystallization and high-resolution structure determination. Flexibility of the linker region allows for different relative orientations of barnase and the fusion partner in two crystallographically independent molecules and may thereby facilitate crystal packing. Nevertheless, the linker region is well ordered in both molecules. This system may prove more generally useful to determine the crystal structure of peptides and small proteins.

Barnase fusion as a tool to determine the crystal structure of the small disulfide-rich protein McoEeTI.,Niemann HH, Schmoldt HU, Wentzel A, Kolmar H, Heinz DW J Mol Biol. 2006 Feb 10;356(1):1-8. Epub 2005 Nov 21. PMID:16337652^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Niemann HH, Schmoldt HU, Wentzel A, Kolmar H, Heinz DW. Barnase fusion as a tool to determine the crystal structure of the small disulfide-rich protein McoEeTI. J Mol Biol. 2006 Feb 10;356(1):1-8. Epub 2005 Nov 21. PMID:16337652 doi:10.1016/j.jmb.2005.11.005

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2c4b"

@@ Line 1: / Line 1: @@
-==INHIBITOR CYSTINE KNOT PROTEIN MCOEETI FUSED TO THE CATALYTICALLY INACTIVE BARNASE MUTANT H102A==
+==Inhibitor cystine knot protein McoEeTI fused to the catalytically inactive barnase mutant H102A==
 <StructureSection load='2c4b' size='340' side='right' caption='[[2c4b]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2c4b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C4B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C4B FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2c4b]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C4B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C4B FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1a2p|1a2p]], [[1b20|1b20]], [[1b21|1b21]], [[1b27|1b27]], [[1b2s|1b2s]], [[1b2u|1b2u]], [[1b2x|1b2x]], [[1b2z|1b2z]], [[1b3s|1b3s]], [[1ban|1ban]], [[1bao|1bao]], [[1bgs|1bgs]], [[1bne|1bne]], [[1bnf|1bnf]], [[1bng|1bng]], [[1bni|1bni]], [[1bnj|1bnj]], [[1bnr|1bnr]], [[1bns|1bns]], [[1brg|1brg]], [[1brh|1brh]], [[1bri|1bri]], [[1brj|1brj]], [[1brk|1brk]], [[1brn|1brn]], [[1brs|1brs]], [[1bsa|1bsa]], [[1bsb|1bsb]], [[1bsc|1bsc]], [[1bsd|1bsd]], [[1bse|1bse]], [[1fw7|1fw7]], [[1h9h|1h9h]], [[1h9i|1h9i]], [[1ha9|1ha9]], [[1ib9|1ib9]], [[1rnb|1rnb]], [[1w7z|1w7z]], [[1x1u|1x1u]], [[1x1w|1x1w]], [[1x1x|1x1x]], [[1x1y|1x1y]], [[1yvs|1yvs]], [[2eti|2eti]], [[2let|2let]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c4b OCA], [http://pdbe.org/2c4b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2c4b RCSB], [http://www.ebi.ac.uk/pdbsum/2c4b PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c4b OCA], [http://pdbe.org/2c4b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2c4b RCSB], [http://www.ebi.ac.uk/pdbsum/2c4b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2c4b ProSAT]</span></td></tr>
 </table>
 == Function ==

2c4b

From Proteopedia

Revision as of 13:16, 15 March 2017

Inhibitor cystine knot protein McoEeTI fused to the catalytically inactive barnase mutant H102A

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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