5t5a
From Proteopedia
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t5a OCA], [http://pdbe.org/5t5a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t5a RCSB], [http://www.ebi.ac.uk/pdbsum/5t5a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t5a ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t5a OCA], [http://pdbe.org/5t5a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t5a RCSB], [http://www.ebi.ac.uk/pdbsum/5t5a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t5a ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The TS ribozyme (originally called "twister sister") is a catalytic RNA. We present a crystal structure of the ribozyme in a pre-reactive conformation. Two co-axial helical stacks are organized by a three-way junction and two tertiary contacts. Five divalent metal ions are directly coordinated to RNA ligands, making important contributions to the RNA architecture. The scissile phosphate lies in a quasihelical loop region that is organized by a network of hydrogen bonding. A divalent metal ion is directly bound to the nucleobase 5' to the scissile phosphate, with an inner-sphere water molecule positioned to interact with the O2' nucleophile. The rate of ribozyme cleavage correlated in a log-linear manner with divalent metal ion pKa, consistent with proton transfer in the transition state, and we propose that the bound metal ion is a likely general base for the cleavage reaction. Our data indicate that the TS ribozyme functions predominantly as a metalloenzyme. | ||
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| + | The structure of a nucleolytic ribozyme that employs a catalytic metal ion.,Liu Y, Wilson TJ, Lilley DM Nat Chem Biol. 2017 Mar 6. doi: 10.1038/nchembio.2333. PMID:28263963<ref>PMID:28263963</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5t5a" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 13:31, 15 March 2017
Crystal Structure of the Twister Sister (TS) Ribozyme at 2.0 Angstrom
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