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1v04

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Revision as of 13:41, 15 March 2017

serum paraoxonase by directed evolution

Structural highlights

1v04 is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Members of the serum paraoxonase (PON) family have been identified in mammals and other vertebrates, and in invertebrates. PONs exhibit a wide range of physiologically important hydrolytic activities, including drug metabolism and detoxification of nerve agents. PON1 and PON3 reside on high-density lipoprotein (HDL, 'good cholesterol') and are involved in the prevention of atherosclerosis. We describe the first crystal structure of a PON family member, a variant of PON1 obtained by directed evolution, at a resolution of 2.2 A. PON1 is a six-bladed beta-propeller with a unique active site lid that is also involved in HDL binding. The three-dimensional structure and directed evolution studies permit a detailed description of PON1's active site and catalytic mechanism, which are reminiscent of secreted phospholipase A2, and of the routes by which PON family members diverged toward different substrate and reaction selectivities.

Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzymes.,Harel M, Aharoni A, Gaidukov L, Brumshtein B, Khersonsky O, Meged R, Dvir H, Ravelli RB, McCarthy A, Toker L, Silman I, Sussman JL, Tawfik DS Nat Struct Mol Biol. 2004 May;11(5):412-9. Epub 2004 Apr 18. PMID:15098021^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Harel M, Aharoni A, Gaidukov L, Brumshtein B, Khersonsky O, Meged R, Dvir H, Ravelli RB, McCarthy A, Toker L, Silman I, Sussman JL, Tawfik DS. Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzymes. Nat Struct Mol Biol. 2004 May;11(5):412-9. Epub 2004 Apr 18. PMID:15098021 doi:10.1038/nsmb767

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1v04"

@@ Line 1: / Line 1: @@
-==SERUM PARAOXONASE BY DIRECTED EVOLUTION==
+==serum paraoxonase by directed evolution==
 <StructureSection load='1v04' size='340' side='right' caption='[[1v04]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1v04]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens,_oryctolagus_cuniculus,_mus_musculus,_rattus_rattus Homo sapiens, oryctolagus cuniculus, mus musculus, rattus rattus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V04 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1V04 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1v04]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V04 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1V04 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1v04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v04 OCA], [http://pdbe.org/1v04 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1v04 RCSB], [http://www.ebi.ac.uk/pdbsum/1v04 PDBsum], [http://www.topsan.org/Proteins/ISPC/1v04 TOPSAN]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1v04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v04 OCA], [http://pdbe.org/1v04 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1v04 RCSB], [http://www.ebi.ac.uk/pdbsum/1v04 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1v04 ProSAT], [http://www.topsan.org/Proteins/ISPC/1v04 TOPSAN]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 14: / Line 15: @@
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1v04 ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 34: / Line 35: @@
 __TOC__
 </StructureSection>
-[[Category: Homo sapiens, oryctolagus cuniculus, mus musculus, rattus rattus]]
 [[Category: Aharoni, A]]
 [[Category: Brumshtein, B]]
@@ Line 41: / Line 41: @@
 [[Category: Harel, M]]
 [[Category: Khersonsky, O]]
-[[Category: Mccarthy, A]]
+[[Category: McCarthy, A]]
 [[Category: Meged, R]]
 [[Category: Ravelli, R B.G]]

1v04

From Proteopedia

Revision as of 13:41, 15 March 2017

serum paraoxonase by directed evolution

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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