1h0h
From Proteopedia
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[[Category: tungsten selenium formate dehydrogenase]] | [[Category: tungsten selenium formate dehydrogenase]] | ||
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Revision as of 14:19, 5 November 2007
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TUNGSTEN CONTAINING FORMATE DEHYDROGENASE FROM DESULFOVIBRIO GIGAS
Overview
Desulfovibrio gigas formate dehydrogenase is the first representative of a, tungsten-containing enzyme from a mesophile that has been structurally, characterized. It is a heterodimer of 110 and 24 kDa subunits. The large, subunit, homologous to E. coli FDH-H and to D. desulfuricans nitrate, reductase, harbors the W site and one [4Fe-4S] center. No small subunit, ortholog containing three [4Fe-4S] clusters has been reported. The, structural homology with E. coli FDH-H shows that the essential residues, (SeCys158, His159, and Arg407) at the active site are conserved. The, active site is accessible via a positively charged tunnel, while product, release may be facilitated, for H(+) by buried waters and protonable amino, acids and for CO(2) through a hydrophobic channel.
About this Structure
1H0H is a Protein complex structure of sequences from Desulfovibrio gigas with CA, 2MD, MGD, S, SF4 and EPE as ligands. Structure known Active Site: WA. Full crystallographic information is available from OCA.
Reference
Gene sequence and the 1.8 A crystal structure of the tungsten-containing formate dehydrogenase from Desulfovibrio gigas., Raaijmakers H, Macieira S, Dias JM, Teixeira S, Bursakov S, Huber R, Moura JJ, Moura I, Romao MJ, Structure. 2002 Sep;10(9):1261-72. PMID:12220497
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