1tpz
From Proteopedia
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|PDB= 1tpz |SIZE=350|CAPTION= <scene name='initialview01'>1tpz</scene>, resolution 2.0Å | |PDB= 1tpz |SIZE=350|CAPTION= <scene name='initialview01'>1tpz</scene>, resolution 2.0Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= IIGP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | |GENE= IIGP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1tqd|1TQD]], [[1tq2|1TQ2]], [[1tq4|1TQ4]], [[1tq6|1TQ6]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tpz OCA], [http://www.ebi.ac.uk/pdbsum/1tpz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1tpz RCSB]</span> | ||
}} | }} | ||
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[[Category: Uthaiah, R.]] | [[Category: Uthaiah, R.]] | ||
[[Category: Wolf, E.]] | [[Category: Wolf, E.]] | ||
| - | [[Category: EDO]] | ||
| - | [[Category: GDP]] | ||
| - | [[Category: MG]] | ||
| - | [[Category: MPD]] | ||
[[Category: crystal structure]] | [[Category: crystal structure]] | ||
[[Category: dimer]] | [[Category: dimer]] | ||
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[[Category: interferon gamma]] | [[Category: interferon gamma]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:59:31 2008'' |
Revision as of 20:59, 30 March 2008
| |||||||
| , resolution 2.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Gene: | IIGP1 (Mus musculus) | ||||||
| Related: | 1TQD, 1TQ2, 1TQ4, 1TQ6
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of IIGP1: a paradigm for interferon inducible p47 resistance GTPases
Overview
Interferon-inducible p47 GTPases are critical mediators of cell-autonomous resistance against several intracellular pathogens. Here we present the first crystal structure of a member of this novel GTPase family, IIGP1, in its nucleotide-free, GDP-, and GppNHp-bound form. The structure shows a Ras-like G domain between an N-terminal three-helix bundle and a complex system of C-terminal helices and loops. Sequence comparison and secondary structure prediction suggest the IIGP1 structure to be a valid model for the p47 GTPase family. The IIGP1 crystals contain a noncrystallographic dimer. We show that the dimer is required for cooperative GTP hydrolysis and GTP-dependent oligomerization of IIGP1. We also present the GDP- and GppNHp-bound monomeric structures of two dimer interface mutants. Our structures direct approaches to the analysis of the catalytic mechanism of IIGP1 and provide a coherent basis for structure-function studies aimed at elucidating the mechanistic basis of pathogen resistance caused by these enigmatic GTPases.
About this Structure
1TPZ is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Crystal structure of IIGP1: a paradigm for interferon-inducible p47 resistance GTPases., Ghosh A, Uthaiah R, Howard J, Herrmann C, Wolf E, Mol Cell. 2004 Sep 10;15(5):727-39. PMID:15350217
Page seeded by OCA on Sun Mar 30 23:59:31 2008
Categories: Mus musculus | Single protein | Ghosh, A. | Herrmann, C. | Howard, J. | Uthaiah, R. | Wolf, E. | Crystal structure | Dimer | Gtpase | Immunology | Interferon gamma
