1tu3
From Proteopedia
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|PDB= 1tu3 |SIZE=350|CAPTION= <scene name='initialview01'>1tu3</scene>, resolution 2.31Å | |PDB= 1tu3 |SIZE=350|CAPTION= <scene name='initialview01'>1tu3</scene>, resolution 2.31Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= RAB5A, RAB5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), RABEP1, RABPT5, RABPT5A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= RAB5A, RAB5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), RABEP1, RABPT5, RABPT5A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tu3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tu3 OCA], [http://www.ebi.ac.uk/pdbsum/1tu3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1tu3 RCSB]</span> | ||
}} | }} | ||
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[[Category: Zhang, X C.]] | [[Category: Zhang, X C.]] | ||
[[Category: Zhu, G.]] | [[Category: Zhu, G.]] | ||
| - | [[Category: GNP]] | ||
| - | [[Category: MG]] | ||
[[Category: effector-binding]] | [[Category: effector-binding]] | ||
[[Category: rab5]] | [[Category: rab5]] | ||
[[Category: rabaptin5]] | [[Category: rabaptin5]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:01:07 2008'' |
Revision as of 21:01, 30 March 2008
| |||||||
| , resolution 2.31Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | RAB5A, RAB5 (Homo sapiens), RABEP1, RABPT5, RABPT5A (Homo sapiens) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Rab5 complex with Rabaptin5 C-terminal Domain
Overview
Rab5 is a small GTPase that regulates early endosome fusion. We present here the crystal structure of the Rab5 GTPase domain in complex with a GTP analog and the C-terminal domain of effector Rabaptin5. The proteins form a dyad-symmetric Rab5-Rabaptin5(2)-Rab5 ternary complex with a parallel coiled-coil Rabaptin5 homodimer in the middle. Two Rab5 molecules bind independently to the Rabaptin5 dimer using their switch and interswitch regions. The binding does not involve the Rab complementarity-determining regions. We also present the crystal structures of two distinct forms of GDP-Rab5 complexes, both of which are incompatible with Rabaptin5 binding. One has a dislocated and disordered switch I but a virtually intact switch II, whereas the other has its beta-sheet and both switch regions reorganized. Biochemical and functional analyses show that the crystallographically observed Rab5-Rabaptin5 complex also exists in solution, and disruption of this complex by mutation abrogates endosome fusion.
About this Structure
1TU3 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis of Rab5-Rabaptin5 interaction in endocytosis., Zhu G, Zhai P, Liu J, Terzyan S, Li G, Zhang XC, Nat Struct Mol Biol. 2004 Oct;11(10):975-83. Epub 2004 Sep 19. PMID:15378032
Page seeded by OCA on Mon Mar 31 00:01:07 2008
Categories: Homo sapiens | Protein complex | Li, G. | Liu, J. | Terzyan, S. | Zhai, P. | Zhang, X C. | Zhu, G. | Effector-binding | Rab5 | Rabaptin5
