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1tv3
From Proteopedia
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|PDB= 1tv3 |SIZE=350|CAPTION= <scene name='initialview01'>1tv3</scene>, resolution 2.20Å | |PDB= 1tv3 |SIZE=350|CAPTION= <scene name='initialview01'>1tv3</scene>, resolution 2.20Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=BG4:5-(HYDROXY-METHYL-AMINO)-3-METHYL-PYRROLIDINE-2-CARBOXYLIC ACID'>BG4</scene> | + | |LIGAND= <scene name='pdbligand=BG4:5-(HYDROXY-METHYL-AMINO)-3-METHYL-PYRROLIDINE-2-CARBOXYLIC+ACID'>BG4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1nth|1NTH]], [[1l2q|1L2Q]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tv3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tv3 OCA], [http://www.ebi.ac.uk/pdbsum/1tv3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1tv3 RCSB]</span> | ||
}} | }} | ||
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[[Category: Soares, J A.]] | [[Category: Soares, J A.]] | ||
[[Category: Zhao, G.]] | [[Category: Zhao, G.]] | ||
| - | [[Category: BG4]] | ||
[[Category: tim barrel]] | [[Category: tim barrel]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:01:35 2008'' |
Revision as of 21:01, 30 March 2008
| |||||||
| , resolution 2.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Related: | 1NTH, 1L2Q
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the N-methyl-hydroxylamine MtmB complex
Overview
L-pyrrolysine, the 22(nd) genetically encoded amino acid, was previously deduced to be (4R, 5R)-4-substituted-pyrroline-5-carboxylate attached to the epsilon-nitrogen of lysine based on the crystal structure of the M. barkeri monomethylamine methyltransferase (MtmB). To confirm L-pyrrolysine's identity, structures of MtmB have been determined following treatment with hydroxylamine, N-methylhydroxylamine, or dithionite. Analysis of these structures has provided additional support for the presence of the pyrroline ring and, together with previous mass spectroscopy data, has led us to assign the C(4)-substituent to a methyl group. Based on this assignment, synthetic L-pyrrolysine was prepared by chemical methods. Detailed study of this chemically synthesized L-pyrrolysine has allowed us to characterize its physical properties, to study its chemical stability, and to elucidate the role of its C(4) substituent. Future applications of this synthetic L-pyrrolysine include its in vivo incorporation into recombinant proteins.
About this Structure
1TV3 is a Single protein structure of sequence from Methanosarcina barkeri. Full crystallographic information is available from OCA.
Reference
Reactivity and chemical synthesis of L-pyrrolysine- the 22(nd) genetically encoded amino acid., Hao B, Zhao G, Kang PT, Soares JA, Ferguson TK, Gallucci J, Krzycki JA, Chan MK, Chem Biol. 2004 Sep;11(9):1317-24. PMID:15380192
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