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5mnd
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==SFX structure of Cydia pomonella granulovirus using a double flow-focusing nozzle== | |
| + | <StructureSection load='5mnd' size='340' side='right' caption='[[5mnd]], [[Resolution|resolution]] 2.56Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5mnd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cydia_pomonella_granulosis_virus_(isolate_mexico/1963) Cydia pomonella granulosis virus (isolate mexico/1963)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MND OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MND FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mnd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mnd OCA], [http://pdbe.org/5mnd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mnd RCSB], [http://www.ebi.ac.uk/pdbsum/5mnd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mnd ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/GRAN_GVCPM GRAN_GVCPM]] Component of the virus occlusion bodies, which are large proteinaceous structures, that protect the virus from the outside environment for extended periods until they are ingested by insect larvae. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices. | ||
| - | + | Double-flow focused liquid injector for efficient serial femtosecond crystallography.,Oberthuer D, Knoska J, Wiedorn MO, Beyerlein KR, Bushnell DA, Kovaleva EG, Heymann M, Gumprecht L, Kirian RA, Barty A, Mariani V, Tolstikova A, Adriano L, Awel S, Barthelmess M, Dorner K, Xavier PL, Yefanov O, James DR, Nelson G, Wang D, Calvey G, Chen Y, Schmidt A, Szczepek M, Frielingsdorf S, Lenz O, Snell E, Robinson PJ, Sarler B, Belsak G, Macek M, Wilde F, Aquila A, Boutet S, Liang M, Hunter MS, Scheerer P, Lipscomb JD, Weierstall U, Kornberg RD, Spence JC, Pollack L, Chapman HN, Bajt S Sci Rep. 2017 Mar 16;7:44628. doi: 10.1038/srep44628. PMID:28300169<ref>PMID:28300169</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| + | <div class="pdbe-citations 5mnd" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Chapman, H]] | [[Category: Chapman, H]] | ||
| - | [[Category: Oberthuer, D]] | ||
[[Category: Doerner, K]] | [[Category: Doerner, K]] | ||
| - | [[Category: Xavier, P | + | [[Category: Oberthuer, D]] |
| + | [[Category: Xavier, P L]] | ||
| + | [[Category: Granulovirus]] | ||
| + | [[Category: In vivo crystal]] | ||
| + | [[Category: Nanocrystal]] | ||
| + | [[Category: Sfx]] | ||
| + | [[Category: Viral protein]] | ||
Revision as of 13:47, 29 March 2017
SFX structure of Cydia pomonella granulovirus using a double flow-focusing nozzle
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