1u5w

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|PDB= 1u5w |SIZE=350|CAPTION= <scene name='initialview01'>1u5w</scene>, resolution 2.3&Aring;
|PDB= 1u5w |SIZE=350|CAPTION= <scene name='initialview01'>1u5w</scene>, resolution 2.3&Aring;
|SITE=
|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
|ACTIVITY=
|ACTIVITY=
|GENE= yjjX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
|GENE= yjjX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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|DOMAIN=
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u5w OCA], [http://www.ebi.ac.uk/pdbsum/1u5w PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u5w RCSB]</span>
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}}
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[[Category: Singh, V K.]]
[[Category: Singh, V K.]]
[[Category: Zheng, J.]]
[[Category: Zheng, J.]]
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[[Category: SO4]]
 
[[Category: 3 layers alpha/beta/alpha protein]]
[[Category: 3 layers alpha/beta/alpha protein]]
[[Category: bsgi]]
[[Category: bsgi]]
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[[Category: structural genomic]]
[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:27:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:05:45 2008''

Revision as of 21:05, 30 March 2008


PDB ID 1u5w

Drag the structure with the mouse to rotate
, resolution 2.3Å
Ligands:
Gene: yjjX (Escherichia coli)
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal structure of hypothetical protein yjjX from Escherichia coli


Overview

Inosine triphosphate (ITP) and xanthosine triphosphate (XTP) are formed upon deamination of ATP and GTP as a result of exposure to chemical mutagens and oxidative damage. Nucleic acid synthesis requires safeguard mechanisms to minimize undesired lethal incorporation of ITP and XTP. Here, we present the crystal structure of YjjX, a protein of hitherto unknown function. The three-dimensional fold of YjjX is similar to those of Mj0226 from Methanococcus janschii, which possesses nucleotidase activity, and of Maf from Bacillus subtilis, which can bind nucleotides. Biochemical analyses of YjjX revealed it to exhibit specific phosphatase activity for inosine and xanthosine triphosphates and have a possible interaction with elongation factor Tu. The enzymatic activity of YjjX as an inosine/xanthosine triphosphatase provides evidence for a plausible protection mechanism by clearing the noncanonical nucleotides from the cell during oxidative stress in E. coli.

About this Structure

1U5W is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Identification of an ITPase/XTPase in Escherichia coli by structural and biochemical analysis., Zheng J, Singh VK, Jia Z, Structure. 2005 Oct;13(10):1511-20. PMID:16216582

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