1u73
From Proteopedia
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|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= | ||
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1umv|1umv]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u73 OCA], [http://www.ebi.ac.uk/pdbsum/1u73 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u73 RCSB]</span> | ||
}} | }} | ||
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[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:06:11 2008'' |
Revision as of 21:06, 30 March 2008
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| , resolution 1.9Å | |||||||
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| Activity: | Phospholipase A(2), with EC number 3.1.1.4 | ||||||
| Related: | 1umv
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a Dimeric Acidic Platelet Aggregation Inhibitor and Hypotensive Phospholipase A2 from Bothrops jararacussu
Overview
Phospholipases A2 belong to the superfamily of proteins which hydrolyzes the sn-2 acyl groups of membrane phospholipids to release arachidonic acid and lysophospholipids. An acidic phospholipase A2 isolated from Bothrops jararacussu snake venom presents a high catalytic, platelet aggregation inhibition and hypotensive activities. This protein was crystallized in two oligomeric states: monomeric and dimeric. The crystal structures were solved at 1.79 and 1.90 angstroms resolution, respectively, for the two states. It was identified a Na+ ion at the center of Ca2+-binding site of the monomeric form. A novel dimeric conformation with the active sites exposed to the solvent was observed. Conformational states of the molecule may be due to the physicochemical conditions used in the crystallization experiments. We suggest dimeric state is one found in vivo.
About this Structure
1U73 is a Single protein structure of sequence from Bothrops jararacussu. Full crystallographic information is available from OCA.
Reference
Crystal structure of an acidic platelet aggregation inhibitor and hypotensive phospholipase A2 in the monomeric and dimeric states: insights into its oligomeric state., Magro AJ, Murakami MT, Marcussi S, Soares AM, Arni RK, Fontes MR, Biochem Biophys Res Commun. 2004 Oct 8;323(1):24-31. PMID:15351695
Page seeded by OCA on Mon Mar 31 00:06:11 2008
Categories: Bothrops jararacussu | Phospholipase A(2) | Single protein | Arni, R K. | Fontes, M R. | Magro, A J. | Murakami, M T. | Soares, A M. | Acidic phospholipase a2 | Bothrops jararacussu venom | Crystal structure | Dimeric | Oligomeric state | Platelet aggregation and hypotensive effect | X-ray crystallography
