1u7w
From Proteopedia
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|PDB= 1u7w |SIZE=350|CAPTION= <scene name='initialview01'>1u7w</scene>, resolution 2.5Å | |PDB= 1u7w |SIZE=350|CAPTION= <scene name='initialview01'>1u7w</scene>, resolution 2.5Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CTP:CYTIDINE-5'-TRIPHOSPHATE'>CTP</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Phosphopantothenate--cysteine_ligase Phosphopantothenate--cysteine ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.5 6.3.2.5] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopantothenate--cysteine_ligase Phosphopantothenate--cysteine ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.5 6.3.2.5] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1u7u|1U7U]], [[1u7z|1U7Z]], [[1u80|1U80]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u7w OCA], [http://www.ebi.ac.uk/pdbsum/1u7w PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u7w RCSB]</span> | ||
}} | }} | ||
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[[Category: Stanitzek, S.]] | [[Category: Stanitzek, S.]] | ||
[[Category: Steinbacher, S.]] | [[Category: Steinbacher, S.]] | ||
| - | [[Category: CA]] | ||
| - | [[Category: CTP]] | ||
[[Category: coenzyme a biosynthesis]] | [[Category: coenzyme a biosynthesis]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:06:36 2008'' |
Revision as of 21:06, 30 March 2008
| |||||||
| , resolution 2.5Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | Phosphopantothenate--cysteine ligase, with EC number 6.3.2.5 | ||||||
| Related: | 1U7U, 1U7Z, 1U80
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Phosphopantothenoylcysteine synthetase from E. coli, CTP-complex
Overview
Phosphopantothenoylcysteine (PPC) synthetase forms a peptide bond between 4'-phosphopantothenate and cysteine in coenzyme A biosynthesis. PPC synthetases fall into two classes: eukaryotic, ATP-dependent and eubacterial, CTP-dependent enzymes. We describe the first crystal structure of E. coli PPC synthetase as a prototype of bacterial, CTP-dependent PPC synthetases. Structures of the apo-form and the synthetase complexed with CTP, the activated acyl-intermediate, 4'-phosphopantothenoyl-CMP, and with the reaction product CMP provide snapshots along the reaction pathway and detailed insight into substrate binding and the reaction mechanism of peptide bond formation. Binding of the phosphopantothenate moiety of the acyl-intermediate in a cleft at the C-terminal end of the central beta sheet of the dinucleotide binding fold is accomplished by an otherwise flexible flap. A second disordered loop may control access of cysteine to the active site. The conservation of functionalities involved in substrate binding and catalysis provides insight into similarities and differences of prokaryotic and eukaryotic PPC synthetases.
About this Structure
1U7W is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural basis of CTP-dependent peptide bond formation in coenzyme A biosynthesis catalyzed by Escherichia coli PPC synthetase., Stanitzek S, Augustin MA, Huber R, Kupke T, Steinbacher S, Structure. 2004 Nov;12(11):1977-88. PMID:15530362
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