1u8d
From Proteopedia
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|PDB= 1u8d |SIZE=350|CAPTION= <scene name='initialview01'>1u8d</scene>, resolution 1.95Å | |PDB= 1u8d |SIZE=350|CAPTION= <scene name='initialview01'>1u8d</scene>, resolution 1.95Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=HPA:HYPOXANTHINE'>HPA</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE+ION'>NCO</scene>, <scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u8d OCA], [http://www.ebi.ac.uk/pdbsum/1u8d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u8d RCSB]</span> | ||
}} | }} | ||
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[[Category: Gilbert, S D.]] | [[Category: Gilbert, S D.]] | ||
[[Category: Montange, R K.]] | [[Category: Montange, R K.]] | ||
| - | [[Category: ACT]] | ||
| - | [[Category: HPA]] | ||
| - | [[Category: NCO]] | ||
| - | [[Category: SPD]] | ||
[[Category: base quadruple]] | [[Category: base quadruple]] | ||
[[Category: base triple]] | [[Category: base triple]] | ||
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[[Category: rna-ligand complex]] | [[Category: rna-ligand complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:06:42 2008'' |
Revision as of 21:06, 30 March 2008
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| , resolution 1.95Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Guanine riboswitch bound to hypoxanthine
Overview
Riboswitches are genetic regulatory elements found in the 5' untranslated region of messenger RNA that act in the absence of protein cofactors. They are broadly distributed across bacteria and account for the regulation of more than 2% of all genes in Bacillus subtilis, underscoring their importance in the control of cellular metabolism. The 5' untranslated region of many mRNAs of genes involved in purine metabolism and transport contain a guanine-responsive riboswitch that directly binds guanine, hypoxanthine or xanthine to terminate transcription. Here we report the crystal structure at 1.95 A resolution of the purine-binding domain of the guanine riboswitch from the xpt-pbuX operon of B. subtilis bound to hypoxanthine, a prevalent metabolite in the bacterial purine salvage pathway. This structure reveals a complex RNA fold involving several phylogenetically conserved nucleotides that create a binding pocket that almost completely envelops the ligand. Hypoxanthine functions to stabilize this structure and to promote the formation of a downstream transcriptional terminator element, thereby providing a mechanism for directly repressing gene expression in response to an increase in intracellular concentrations of metabolite.
About this Structure
1U8D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure of a natural guanine-responsive riboswitch complexed with the metabolite hypoxanthine., Batey RT, Gilbert SD, Montange RK, Nature. 2004 Nov 18;432(7015):411-5. PMID:15549109
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