5k7t
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==MicroED structure of thermolysin at 2.5 A resolution== | |
| + | <StructureSection load='5k7t' size='340' side='right' caption='[[5k7t]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5k7t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K7T FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5k7n|5k7n]], [[5k7o|5k7o]], [[5k7p|5k7p]], [[5k7q|5k7q]], [[5k7r|5k7r]], [[5k7s|5k7s]]</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k7t OCA], [http://pdbe.org/5k7t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k7t RCSB], [http://www.ebi.ac.uk/pdbsum/5k7t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k7t ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/THER_BACTH THER_BACTH]] Extracellular zinc metalloprotease. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography. | ||
| - | + | Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.,de la Cruz MJ, Hattne J, Shi D, Seidler P, Rodriguez J, Reyes FE, Sawaya MR, Cascio D, Weiss SC, Kim SK, Hinck CS, Hinck AP, Calero G, Eisenberg D, Gonen T Nat Methods. 2017 Feb 13;14(4):399-402. doi: 10.1038/nmeth.4178. PMID:28192420<ref>PMID:28192420</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5k7t" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bacillus thermoproteolyticus]] | ||
| + | [[Category: Thermolysin]] | ||
| + | [[Category: Cascio, D]] | ||
| + | [[Category: Cruz, M J.de la]] | ||
| + | [[Category: Eisenberg, D]] | ||
| + | [[Category: Gonen, T]] | ||
| + | [[Category: Hattne, J]] | ||
| + | [[Category: Reyes, F E]] | ||
| + | [[Category: Rodriguez, J]] | ||
| + | [[Category: Sawaya, M R]] | ||
| + | [[Category: Seidler, P]] | ||
| + | [[Category: Shi, D]] | ||
| + | [[Category: Hydrolase]] | ||
Revision as of 13:19, 5 April 2017
MicroED structure of thermolysin at 2.5 A resolution
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