1u8t
From Proteopedia
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|PDB= 1u8t |SIZE=350|CAPTION= <scene name='initialview01'>1u8t</scene>, resolution 1.50Å | |PDB= 1u8t |SIZE=350|CAPTION= <scene name='initialview01'>1u8t</scene>, resolution 1.50Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | + | |LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= cheY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= cheY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[3chy|3CHY]], [[1fqw|1FQW]], [[1f4v|1F4V]], [[1ehc|1EHC]], [[5chy|5CHY]], [[1d4z|1D4Z]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u8t OCA], [http://www.ebi.ac.uk/pdbsum/1u8t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u8t RCSB]</span> | ||
}} | }} | ||
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[[Category: Quillin, M L.]] | [[Category: Quillin, M L.]] | ||
[[Category: Westbrook, E M.]] | [[Category: Westbrook, E M.]] | ||
| - | [[Category: SO4]] | ||
[[Category: (beta/alpha)5]] | [[Category: (beta/alpha)5]] | ||
[[Category: chey]] | [[Category: chey]] | ||
[[Category: flim]] | [[Category: flim]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:06:55 2008'' |
Revision as of 21:06, 30 March 2008
| |||||||
| , resolution 1.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | cheY (Escherichia coli) | ||||||
| Related: | 3CHY, 1FQW, 1F4V, 1EHC, 5CHY, 1D4Z
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of CheY D13K Y106W alone and in complex with a FliM peptide
Overview
CheY is a member of the response regulator protein superfamily that controls the chemotactic swimming response of motile bacteria. The CheY double mutant D13K Y106W (CheY**) is resistant to phosphorylation, yet is a highly effective mimic of phosphorylated CheY in vivo and in vitro. The conformational attributes of this protein that enable it to signal in a phosphorylation-independent manner are unknown. We have solved the crystal structure of selenomethionine-substituted CheY** in the presence of its target, a peptide (FliM16) derived from the flagellar motor switch, FliM, to 1.5A resolution with an R-factor of 19.6%. The asymmetric unit contains four CheY** molecules, two with FliM16 bound, and two without. The two CheY** molecules in the asymmetric unit that are bound to FliM16 adopt a conformation similar to BeF3- -activated wild-type CheY, and also bind FliM16 in a nearly identical manner. The CheY** molecules that do not bind FliM16 are found in a conformation similar to unphosphorylated wild-type CheY, suggesting that the active phenotype of this mutant is enabled by a facile interconversion between the active and inactive conformations. Finally, we propose a ligand-binding model for CheY and CheY**, in which Ile95 changes conformation in a Tyr/Trp106-dependent manner to accommodate FliM.
About this Structure
1U8T is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structure of the constitutively active double mutant CheYD13K Y106W alone and in complex with a FliM peptide., Dyer CM, Quillin ML, Campos A, Lu J, McEvoy MM, Hausrath AC, Westbrook EM, Matsumura P, Matthews BW, Dahlquist FW, J Mol Biol. 2004 Sep 24;342(4):1325-35. PMID:15351654
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