1u90
From Proteopedia
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|PDB= 1u90 |SIZE=350|CAPTION= <scene name='initialview01'>1u90</scene>, resolution 2.00Å | |PDB= 1u90 |SIZE=350|CAPTION= <scene name='initialview01'>1u90</scene>, resolution 2.00Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= Rala, Ral-a, Ral ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9490 Saguinus oedipus]) | |GENE= Rala, Ral-a, Ral ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9490 Saguinus oedipus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1u8y|1U8Y]], [[1u8z|1U8Z]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u90 OCA], [http://www.ebi.ac.uk/pdbsum/1u90 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u90 RCSB]</span> | ||
}} | }} | ||
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[[Category: Nicely, N I.]] | [[Category: Nicely, N I.]] | ||
[[Category: Serrano, V de.]] | [[Category: Serrano, V de.]] | ||
| - | [[Category: GDP]] | ||
| - | [[Category: MG]] | ||
[[Category: gdp]] | [[Category: gdp]] | ||
[[Category: gmppnp]] | [[Category: gmppnp]] | ||
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[[Category: ra]] | [[Category: ra]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:07:01 2008'' |
Revision as of 21:07, 30 March 2008
| |||||||
| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | Rala, Ral-a, Ral (Saguinus oedipus) | ||||||
| Related: | 1U8Y, 1U8Z
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap
Overview
RalA is a GTPase with effectors such as Sec5 and Exo84 in the exocyst complex and RalBP1, a GAP for Rho proteins. We report the crystal structures of Ral-GppNHp and Ral-GDP. Disordered switch I and switch II, located away from crystal contacts, are observed in one of the molecules in the asymmetric unit of the Ral-GppNHp structure. In the other molecule in the asymmetric unit, a second Mg(2+) ion is bound to the GppNHp gamma-phosphate in an environment in which switch I is pulled away from the nucleotide and switch II is found in a tight beta turn. Clustering of conserved residues on the surface of Ral-GppNHp identifies two putative sites for protein-protein interaction. One site is adjacent to switch I. The other is modulated by switch II and is obstructed in Ral-GDP. The Ral structures are discussed in the context of the published structures of the Ral/Sec5 complex, Ras, and Rap.
About this Structure
1U90 is a Single protein structure of sequence from Saguinus oedipus. Full crystallographic information is available from OCA.
Reference
Crystal structures of Ral-GppNHp and Ral-GDP reveal two binding sites that are also present in Ras and Rap., Nicely NI, Kosak J, de Serrano V, Mattos C, Structure. 2004 Nov;12(11):2025-36. PMID:15530367
Page seeded by OCA on Mon Mar 31 00:07:01 2008
Categories: Saguinus oedipus | Single protein | Kosak, J. | Mattos, C. | Nicely, N I. | Serrano, V de. | Gdp | Gmppnp | Gnp | Gppnhp | Gtp | Gtpase | Ral | Ra
